| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AADC |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human DDC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3条关于AKT抗体的参考文献示例(内容基于公开文献概括，非真实引用)：
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1. **文献名称**: "Selective AKT1/2/3 antibody validation in PI3K pathway studies"
**作者**: Smith J et al.
**摘要**: 比较了多种商业AKT亚型抗体(AKT1/2/3)的特异性，通过基因敲除细胞系验证抗体交叉反应性，为选择特异性抗体提供实验依据。
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2. **文献名称**: "Phospho-specific AKT (Ser473) antibody optimization for cancer biomarker detection"
**作者**: Lee H et al.
**摘要**: 评估了不同磷酸化AKT(Ser473)抗体在肿瘤组织免疫组化中的表现，提出磷酸化表位稳定性对检测结果的影响及标准化操作建议。
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3. **文献名称**: "AKT isoform-specific signaling in metabolic regulation: Antibody performance analysis"
**作者**: Garcia-Ruiz C et al.
**摘要**: 分析了AKT1、AKT2亚型特异性抗体在小鼠肝脏组织Western blot中的灵敏度差异，揭示不同亚型在代谢疾病中的功能区分。
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如需真实文献，建议通过PubMed搜索关键词(如“AKT antibody validation”)获取近期研究。
The AKT (Protein Kinase B) family, comprising AKT1. AKT2. and AKT3. plays a central role in the PI3K/AKT/mTOR signaling pathway, regulating critical cellular processes such as survival, proliferation, metabolism, and apoptosis. These serine/threonine kinases are activated by growth factors or hormones via phosphorylation at key residues (e.g., Thr308 and Ser473 in AKT1), which drives their translocation to cellular membranes and subsequent interaction with downstream targets. Dysregulation of AKT signaling is implicated in cancer, diabetes, and neurodegenerative diseases, making AKT a key therapeutic target.
AKT antibodies are essential tools for studying AKT expression, activation, and localization in research and diagnostics. They detect total AKT proteins or specific phosphorylated forms (e.g., p-AKT Ser473) to assess pathway activity. Applications include Western blotting, immunohistochemistry, and flow cytometry. Selective antibodies distinguishing between AKT isoforms help elucidate their distinct roles; for example, AKT1 is linked to growth, AKT2 to glucose metabolism, and AKT3 to brain development. In cancer research, AKT antibodies aid in evaluating oncogenic signaling and drug resistance. However, variability in antibody specificity, epitope recognition, and cross-reactivity requires rigorous validation using knockout controls or phospho-blocking peptides. Clinically, phospho-AKT antibodies may serve as biomarkers for targeted therapy response. Their reliability is critical for advancing AKT-targeted therapies, such as ATP-competitive inhibitors and allosteric modulators.
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