| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP |
| Entrez GeneID | 3309 |
| clone | 7B5 |
| WB Predicted band size | Calculated MW: 72 kDa; Observed MW: 78 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于GRP78/BiP抗体的3篇代表性文献摘要：
1. **文献名称**：GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development
**作者**：Luo S. et al.
**摘要**：研究利用GRP78/BiP抗体在小鼠胚胎中发现GRP78对早期胚胎细胞增殖和抑制内质网应激相关凋亡的关键作用，揭示了其在胚胎发育中的必要性。
2. **文献名称**：Targeting GRP78-dependent antibody-mediated immune suppression in cancer immunotherapy
**作者**：Ni M. et al.
**摘要**：通过GRP78/BiP抗体实验，证明肿瘤微环境中GRP78通过调控免疫抑制性T细胞活性促进免疫逃逸，提示其作为癌症免疫治疗靶点的潜力。
3. **文献名称**：Role of GRP78 in promoting SARS-CoV-2 spike protein internalization and pathogenesis
**作者**：Chu H. et al.
**摘要**：研究利用抗体阻断实验发现GRP78作为新冠病毒刺突蛋白的共受体，介导病毒入侵宿主细胞，为抗病毒治疗提供了新方向。
4. **文献名称**：GRP78 regulates Parkinson’s disease-associated α-synuclein toxicity through protein folding control
**作者**：Yang Y. et al.
**摘要**：通过Western blot和免疫共沉淀技术，证明GRP78/BiP抗体可检测其与α-突触核蛋白的相互作用，揭示其通过纠正错误折叠蛋白减轻帕金森病神经元损伤的机制。
注：以上文献为虚拟示例，实际研究需通过PubMed/Google Scholar检索真实文献(如可参考Lee A.S.团队关于GRP78的经典研究)。建议使用关键词"GRP78/BiP antibody application"或结合具体研究领域筛选近年文献。
GRP78 (Glucose-Regulated Protein 78), also known as BiP (Immunoglobulin Heavy-Chain Binding Protein), is a pivotal molecular chaperone in the endoplasmic reticulum (ER). It belongs to the HSP70 protein family and plays a central role in maintaining ER homeostasis by facilitating protein folding, regulating the unfolded protein response (UPR), and targeting misfolded proteins for degradation. During ER stress, GRP78/BiP dissociates from transmembrane sensors like PERK, IRE1. and ATF6. activating signaling pathways to restore cellular balance. Dysregulation of GRP78/BiP is linked to diseases including cancer, neurodegenerative disorders, diabetes, and viral infections.
Antibodies targeting GRP78/BiP are essential tools for studying its expression, localization, and function. They are widely used in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. In cancer research, these antibodies help investigate GRP78/BiP's role in tumor progression, drug resistance, and metastasis, as its overexpression on the cell surface in stressed tumors correlates with poor prognosis. In neurodegenerative studies, they aid in exploring connections between ER stress and protein aggregation in Alzheimer’s or Parkinson’s disease. Additionally, GRP78/BiP antibodies are used to validate its interaction with viral proteins in infection studies and to develop therapeutic strategies targeting its stress-inducible isoforms. Their specificity and reliability make them critical for both basic research and clinical biomarker analysis.
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