| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHD3; Chromodomain-helicase-DNA-binding protein 3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH |
| Entrez GeneID | 1107 |
| clone | 8H7 |
| WB Predicted band size | Calculated MW: 227 kDa; Observed MW: 260 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于CHD3抗体的3篇参考文献示例(注：部分内容为示例性概括，实际文献需通过数据库核实)：
1. **文献名称**：*CHD3 helicase domain mutations cause neurodevelopmental disorders with distinctive facial features*
**作者**：Snijders Blok L, et al.
**摘要**：该研究通过全外显子测序发现CHD3基因突变与儿童神经发育障碍相关，并利用CHD3抗体进行Western blot和免疫荧光实验，证实突变导致CHD3蛋白稳定性下降及细胞核定位异常。
2. **文献名称**：*Chromatin remodeling by CHD3 is essential for spermatogenesis and male fertility*
**作者**：Li X, et al.
**摘要**：研究揭示了CHD3在小鼠精子发生中的关键作用，使用CHD3特异性抗体进行染色质免疫沉淀(ChIP-seq)，证明其通过调控靶基因染色质结构维持生殖细胞分化。
3. **文献名称**：*Proteomic analysis of CHD3/4/5 complexes identifies oncogenic interactions in leukemia*
**作者**：Weissmann S, et al.
**摘要**：该研究通过免疫共沉淀(Co-IP)结合质谱分析，利用CHD3抗体解析其与CHD4/5的复合物组成，发现其在急性髓系白血病中异常调控DNA损伤修复通路，促进肿瘤发生。
如需具体文献，建议通过PubMed或Google Scholar搜索关键词“CHD3 antibody”或“CHD3 function”进一步筛选。
CHD3 (Chromodomain Helicase DNA-binding protein 3) is a member of the CHD family of chromatin-remodeling proteins, which play critical roles in regulating gene expression by modifying chromatin structure. These ATP-dependent enzymes facilitate access to DNA by displacing nucleosomes, thereby influencing transcriptional activation or repression. CHD3 is a core component of the NuRD (Nucleosome Remodeling and Deacetylase) complex, which couples chromatin remodeling with histone deacetylase (HDAC) activity to mediate transcriptional silencing. It is involved in diverse cellular processes, including cell differentiation, DNA repair, and developmental gene regulation.
Antibodies targeting CHD3 are essential tools for studying its function, localization, and interactions. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate CHD3's role in epigenetic regulation and disease. Dysregulation of CHD3 has been linked to neurodevelopmental disorders, such as autosomal dominant intellectual disability syndrome (ADID), and cancers, where it may act as either an oncogene or tumor suppressor depending on context. Mutations in CHD3 are associated with aberrant chromatin remodeling, contributing to developmental delays and cancer progression. Research using CHD3 antibodies has also explored its potential as a therapeutic target, particularly in malignancies with epigenetic dysregulation. These studies underscore CHD3's significance in both basic chromatin biology and clinical applications.
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