| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; MAPK1; ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK. |
| Entrez GeneID | 5595/5594 |
| clone | 9C7 |
| WB Predicted band size | Calculated MW: 44,42 kDa; Observed MW: 44,42 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide conjugated to KLH. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与ERK1/2抗体相关的经典文献及其摘要概括：
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1. **文献名称**：*MAP kinase pathways activated by stress and inflammatory cytokines*
**作者**：Kyriakis JM, Avruch J
**摘要**：该综述详细描述了ERK1/2(MAPK3/1)在应激和炎症因子激活的信号通路中的作用，强调了ERK1/2抗体在检测磷酸化及总蛋白表达中的关键应用，并讨论了其在细胞增殖、分化及凋亡研究中的重要性。
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2. **文献名称**：*Phospho-specific antibodies: Raising tools for studying ERK1/2 signalling*
**作者**：Wong TW, Jove R
**摘要**：本文开发并验证了针对ERK1/2磷酸化位点(Thr202/Tyr204)的特异性抗体，用于Western blot和免疫荧光分析，揭示了其在肿瘤细胞中动态激活的检测价值，并应用于药物靶点研究。
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3. **文献名称**：*Quantitative analysis of ERK1/2 signaling dynamics using phospho-specific flow cytometry*
**作者**：Fritsche-Guenther C, Witzel F, Sieber A
**摘要**：通过流式细胞术结合ERK1/2磷酸化抗体，定量分析了不同刺激下ERK1/2的激活动力学，揭示了其在单细胞水平的时间依赖性响应差异，为癌症治疗中信号通路的异质性研究提供了方法学支持。
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4. **文献名称**：*Subcellular localization-specific antibody validation in ERK1/2 studies*
**作者**：Roux PP, Blenis J
**摘要**：验证了多种ERK1/2抗体在亚细胞定位(如核转位)检测中的特异性，证明其在研究生长因子诱导的ERK1/2核内信号传导及基因表达调控中的关键作用，强调了抗体选择对实验结果的影响。
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这些文献涵盖了ERK1/2抗体的开发、验证及在信号通路研究中的应用，适用于分子生物学及疾病机制研究。
ERK1/2 (Extracellular Signal-Regulated Kinase 1/2), also known as MAPK3/MAPK1. are serine/threonine kinases central to the MAPK/ERK signaling pathway, which regulates critical cellular processes like proliferation, differentiation, survival, and apoptosis. These kinases are activated by dual phosphorylation at specific threonine and tyrosine residues (Thr202/Tyr204 in ERK1; Thr185/Tyr187 in ERK2) via upstream MAPK kinases (MEK1/2). ERK1/2 antibodies are essential tools for studying this pathway, widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect expression, localization, and activation status.
Two main types of ERK1/2 antibodies are commonly used: **total ERK1/2 antibodies** (recognizing both phosphorylated and non-phosphorylated forms) and **phospho-specific ERK1/2 antibodies** (targeting the active, dual-phosphorylated forms). Total ERK antibodies serve as loading controls, while phospho-antibodies indicate pathway activation. Researchers must validate these antibodies using knockout cell lines or phosphorylation inhibitors to ensure specificity, as cross-reactivity with other MAPKs can occur.
ERK1/2 dysregulation is implicated in cancer, neurological disorders, and cardiovascular diseases, making these antibodies vital for mechanistic studies and drug development. Commercial ERK1/2 antibodies are typically raised in rabbits or mice, with monoclonal and polyclonal options available. Proper sample preparation (e.g., rapid lysis with phosphatase inhibitors) is critical for preserving phosphorylation signals during experiments.
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