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产品描述:人甲状腺结合球蛋白(TBG)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的SERPINA7含量。SERPINA7是一个靶点。它可能在生理病理过程扮演重要角色,目前对其具体背景研究或许与特定疾病相关,研究机制方面可能涉及在体内的信号传导、与其他分子的相互作用等方面,不过因缺乏更多信息,其确切详情还待进一步深入探索。试剂盒检测范围为12.5 ng/mL-200 ng/mL,适用于实验室研究甲状腺功能疾病(如甲亢、甲减、甲状腺炎)的分子机制、激素代谢调控通路探索,或药物开发中靶向TBG的作用效果评估本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 ELISA Kit; Serpin A7 ELISA Kit; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 ELISA Kit; Serpin peptidase inhibitor, clade A, member 7 ELISA Kit; Serpina7 ELISA Kit; T4 binding globulin ELISA Kit; TBG ELISA Kit; TBG, serum ELISA Kit; THBG_HUMAN ELISA Kit; Thyroxin binding globulin ELISA Kit; Thyroxine binding globulin ELISA Kit; Thyroxine-binding globulin ELISA Kit; Thyroxine-binding globulin od serum ELISA Kit
缩写:SERPINA7
Uniprot No.:P05543
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:12.5 ng/mL-200 ng/mL
灵敏度:12.5 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human TBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 91 | ||||
Range % | 85-96 | |||||
1:2 | Average % | 96 | ||||
Range % | 92-105 | |||||
1:4 | Average % | 84 | ||||
Range % | 80-89 | |||||
1:8 | Average % | 91 | ||||
Range % | 85-98 |
回收率:
The recovery of human TBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 91 | 87-95 | ||||
EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2Average 2000.1210.1150.118 1000.3280.3280.328 500.5130.3120.413 251.0150.4970.756 12.52.1162.1212.119 |
货期:3-5 working days
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