| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5' ' -AMP-activated protein kinase subunit beta-1; AMP-activated; noncatalytic; beta-1; AMPK; AMPK beta 1 chain; AMPK subunit beta-1; AMPK-BETA-1; AMPKb; HAMPKb; PRKAB1 |
| Entrez GeneID | 5564 |
| WB Predicted band size | Calculated MW: 30 kDa; Observed MW: 38 kDa |
| Host/Isotype | Mouse IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant Protein of AMPKβ1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于AMPK beta 1(PRKAB1)抗体的3篇参考文献的简要总结：
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1. **文献名称**：*"Characterization of a monoclonal antibody specific for AMPK β1 subunit"*
**作者**：Sakamoto K, et al.
**摘要**：该研究开发了一种针对AMPK β1亚基的单克隆抗体，验证了其特异性(不与β2亚基交叉反应)，并应用于免疫印迹和免疫组化实验，证实β1在肝脏和骨骼肌中的高表达。
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2. **文献名称**：*"AMPK β1 knockdown enhances cisplatin sensitivity in non-small cell lung cancer cells"*
**作者**：Li Y, et al.
**摘要**：通过AMPK β1特异性抗体敲低β1表达，研究显示其增强了肺癌细胞对化疗药物顺铂的敏感性，表明β1在调控癌细胞代谢应激和化疗抵抗中起关键作用。
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3. **文献名称**：*"AMPK β1 regulates mitochondrial fission in cardiac ischemia-reperfusion injury"*
**作者**：Wang S, et al.
**摘要**：利用AMPK β1抗体进行蛋白定位分析，发现心脏缺血再灌注损伤中β1通过调控线粒体分裂蛋白Drp1的活性影响心肌细胞存活，提示其作为潜在治疗靶点。
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(注：以上文献为示例性内容，实际引用需核对真实数据库如PubMed或Google Scholar获取具体信息。)
AMP-activated protein kinase (AMPK) is a crucial energy-sensing heterotrimeric complex composed of α, β, and γ subunits, playing a central role in regulating cellular metabolism. The β subunit (specifically β1. encoded by PRKAB1) serves as a scaffold linking the α catalytic subunit and γ regulatory subunit, while also containing a carbohydrate-binding module that helps anchor AMPK to glycogen particles. AMPK β1 is ubiquitously expressed, particularly in metabolic tissues like liver, muscle, and adipose, where it contributes to maintaining energy homeostasis by responding to low ATP levels through phosphorylation of downstream targets involved in glucose uptake, lipid metabolism, and mitochondrial biogenesis.
Antibodies targeting AMPK β1 are essential tools for investigating its expression, localization, and protein-protein interactions. They enable detection of endogenous β1 protein levels via Western blotting, immunoprecipitation, or immunofluorescence, helping researchers study tissue-specific AMPK activity or alterations in metabolic disorders. These antibodies also facilitate exploration of β1's structural role in AMPK assembly and its regulatory crosstalk with signaling pathways in conditions like obesity, diabetes, and cancer. Validated β1-specific antibodies are particularly valuable given the existence of the β2 isoform (PRKAB2), which shares ~70% amino acid identity but shows differential tissue distribution and functional properties. Proper antibody characterization through knockout controls ensures specificity in distinguishing β1 from β2 and accurately interpreting its metabolic regulatory mechanisms.
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