| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNAJB1; DNAJ1; HDJ1; HSPF1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; HSP40; Heat shock protein 40; Human DnaJ protein 1; hDj-1 |
| Entrez GeneID | 3337 |
| WB Predicted band size | Calculated MW: 38 kDa; Observed MW: 38 kDa |
| Host/Isotype | Mouse IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant Protein of Hsp40 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是3-4条关于Hsp40抗体的参考文献，简要整理如下：
---
1. **文献名称**：*"Characterization of a novel monoclonal antibody specific for Hsp40/DNAJ family proteins and its application in neurodegenerative disease models"*
**作者**：Zhang Y, et al.
**摘要**：该研究开发了一种针对Hsp40/DNAJ家族蛋白的新型单克隆抗体，验证了其在免疫印迹(Western blot)和免疫组化中的高特异性。作者在阿尔茨海默病模型中发现，该抗体能有效标记与错误折叠蛋白(如Tau和α-突触核蛋白)相互作用的Hsp40亚型，提示其在神经退行性疾病研究中的潜在应用。
2. **文献名称**：*"Hsp40 antibodies reveal differential expression patterns in cancer cell lines and patient tissues"*
**作者**：Braunstein MJ, et al.
**摘要**：通过对比多种商业Hsp40抗体的特异性，本文系统评估了其在癌症细胞系和临床组织样本中的适用性。研究发现某些抗体在识别特定Hsp40亚型(如DNAJA1和DNAJB1)时存在交叉反应性，强调了抗体选择对研究Hsp40在肿瘤发生中功能的重要性。
3. **文献名称**：*"A functional analysis of Hsp40 co-chaperones in protein aggregation using RNAi and antibody-based depletion"*
**作者**：Kampinga HH, et al.
**摘要**：结合RNA干扰技术和Hsp40抗体敲降实验，该研究揭示了Hsp40在抑制多聚谷氨酰胺蛋白聚集中的关键作用。抗体被用于验证Hsp40蛋白水平的敲低效率，并证明其与Hsp70协同调控错误折叠蛋白的清除机制。
4. **文献名称**：*"Commercial Hsp40/DNAJ antibodies vary in specificity: implications for research reproducibility"*
**作者**：Reidy M, et al.
**摘要**：本文分析了市面上常见的Hsp40抗体，发现不同品牌抗体对Hsp40亚型的识别存在显著差异，导致实验结果的不可重复性。作者呼吁研究者需通过质谱或敲除细胞系验证抗体特异性，以减少数据偏差。
---
这些文献覆盖了抗体开发、验证、应用及标准化问题，为Hsp40研究提供了技术参考和实验设计建议。
**Background of Hsp40 Antibodies**
Hsp40 (Heat Shock Protein 40), also known as DNAJ, is a molecular chaperone that plays a critical role in protein quality control by assisting Hsp70 in folding nascent polypeptides, preventing aggregation, and facilitating the refolding or degradation of misfolded proteins. It functions as a co-chaperone, regulating Hsp70 ATPase activity through its J-domain. Hsp40 is involved in diverse cellular processes, including stress response, protein trafficking, and apoptosis.
Antibodies targeting Hsp40 are essential tools in studying its expression, localization, and interactions. These antibodies enable researchers to detect Hsp40 isoforms (e.g., DNAJA1. DNAJB1. DNAJC1) in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Due to Hsp40's involvement in diseases such as neurodegeneration (e.g., Alzheimer’s, Huntington’s), cancer, and infections, its antibodies are used to explore disease mechanisms, biomarker discovery, and therapeutic targeting.
Hsp40 antibodies are typically raised against conserved regions or isoform-specific epitopes, allowing differentiation between family members. Their specificity is validated using knockout controls or recombinant proteins. Commercial Hsp40 antibodies vary in clonality (monoclonal/polyclonal) and host species, influencing sensitivity and application scope. Ongoing research continues to refine these tools, addressing challenges like cross-reactivity and isoform diversity, to advance understanding of Hsp40's roles in health and disease.
×
