首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠硒蛋白P(SEPP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SEPP1含量。SEPP1即硒蛋白P，它是含硒量最高的硒蛋白，在维持体内硒稳态等方面发挥重要作用。研究发现它参与抗氧化、抗炎等机制，与动脉粥样硬化、神经系统疾病等多种病理过程相关，是药物研发等领域颇具潜力的靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，本产品适用于科研领域对硒蛋白代谢调控机制的研究。适配多种生物样本类型，尤其适用于探究肝脏疾病模型、营养性硒缺乏或氧化应激相关实验中SEPP1的动态变化，为研究硒元素代谢通路、抗氧化系统功能以及相关疾病分子机制提供可靠的检测工具，广泛应用于基础医学、营养学及分子生物学领域的实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Selenop ELISA Kit; Selp ELISA Kit; Sepp1 ELISA Kit; Selenoprotein P ELISA Kit; SeP) [Cleaved into: Selenoprotein Se-P10; Selenoprotein Se-P6; Selenoprotein Se-P2; Selenoprotein Se-P1] ELISA Kit
缩写：SEPP1
Uniprot No.：P25236
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:2000 | Average % | 105 | |||||
| Range % | 100-110 | ||||||
| 1:4000 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:8000 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
回收率：
| The recovery of rat SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 94-102 | |||||
| EDTA plasma (n=4) | 85 | 80-90 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 50002.5222.4962.5092.354 25001.9492.0231.9861.831 12501.6131.5841.5991.444 6251.1861.1351.1611.006 3120.7540.7210.7380.583 1560.4480.4600.4540.299 780.3150.2970.3060.151 00.1510.1580.155 | |||||||
货期：3-5 working days
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