首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人硒蛋白P(SEPP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、尿液样本中的SEPP1含量。SEPP1 是重要的硒蛋白，在维持体内硒稳态等方面发挥关键作用。研究显示其参与氧化应激、炎症反应等机制。它在心血管疾病、神经系统疾病等病理过程中存在表达异常，通过调控相关信号通路影响疾病发生发展，是潜在治疗靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于研究硒代谢调控机制、氧化应激相关疾病模型构建、药物干预效果评价等科研领域，为探索SEPP1在病理生理过程中的分子作用提供灵敏可靠的技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Selenoprotein P ELISA Kit; Selenoprotein P plasma 1 ELISA Kit; Selp ELISA Kit; SeP ELISA Kit; Sepp1 ELISA Kit; SEPP1_HUMAN ELISA Kit
缩写：SEPP1
Uniprot No.：P49908
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, urine
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 93-105 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:1600 | Average % | 95 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 87-101 | ||||
| EDTA plasma (n=4) | 100 | 94-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4832.4762.4802.39651.8761.8961.8861.8022.51.2701.3041.2871.2031.250.7160.7680.7420.6580.6250.5120.5360.5240.4400.3120.2880.2960.2920.2080.150.2110.2290.2200.13600.0820.0860.084 | ||||||
货期：3-5 working days
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