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产品描述：人精囊蛋白-2(SEMG2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SEMG2含量。人精囊蛋白-2（SEMG2）是一种由精囊腺分泌的蛋白质，主要功能是形成精子周围的凝胶状物质，限制精子运动。SEMG2在灵长动物的性别选择行为中起关键作用，其基因的进化速度受性别选择行为影响，进而影响求偶时的竞争优势。此外，SEMG2在肿瘤免疫逃逸中也发挥作用，其与多种免疫受体结合，抑制T细胞对肿瘤细胞的杀伤。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本产品适用于科研领域中对精液蛋白功能、男性生殖生理机制的研究，或通过检测生物样本中SEMG2水平探索其与生殖系统疾病的潜在关联，为体外实验提供高特异性的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SEMG2 ELISA Kit; Semenogelin-2 ELISA Kit; Semenogelin II ELISA Kit; SGII ELISA Kit
缩写：SEMG2
Uniprot No.：Q02383
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SEMG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 85-94 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | Sep-87 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 85-96 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 87-92 | ||||||
回收率：
| The recovery of human SEMG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 86-96 | |||||
| EDTA plasma (n=4) | 103 | 98-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.4232.5792.5012.39310001.9542.0542.0041.8965001.2651.3021.2841.1762500.7120.7340.7230.6151250.4160.4050.4110.30362.50.2510.2660.2590.15131.250.1760.1830.1800.07200.1070.1080.108 | |||||||
货期：3-5 working days
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