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产品描述：人可溶性CD100(sCD100)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SEMA4D含量。SEMA4D即轴突导向因子4D，是免疫球蛋白超家族成员。其背景上，在免疫调节、血管生成等方面有重要作用。研究机制显示，它通过与受体Plexin - B1等结合，调控细胞迁移、增殖等过程，在肿瘤、神经疾病等领域是潜在治疗靶点。试剂盒检测范围为125 pg/mL-8000 pg/mL，科研人员可利用探索sCD100在炎症反应、肿瘤免疫逃逸、神经轴突导向等机制中的生物学功能，或通过检测疾病模型动物/细胞培养上清中的sCD100水平，为免疫调节相关的基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A8 ELISA Kit; BB 18 ELISA Kit; BB18 ELISA Kit; CD 100 ELISA Kit; CD100 ELISA Kit; CD100 antigen ELISA Kit; Coll 4 ELISA Kit; COLL4 ELISA Kit; Collapsin 4 ELISA Kit; Collapsin4 ELISA Kit; GR3 ELISA Kit; Leukocyte activation antigen CD100 ELISA Kit; M sema G ELISA Kit; MSEMA ELISA Kit; SEM4D_HUMAN ELISA Kit; Sema 4d ELISA Kit; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D ELISA Kit; Sema H ELISA Kit; SEMA J ELISA Kit; Sema4d ELISA Kit; Semacl 2 ELISA Kit; Semacl2 ELISA Kit; SemaH ELISA Kit; SEMAJ ELISA Kit; Semaphorin 4D ELISA Kit; Semaphorin C like 2 ELISA Kit; Semaphorin H ELISA Kit; Semaphorin J ELISA Kit; Semaphorin-4D ELISA Kit; Semaphorin4D ELISA Kit; SemaphorinJ ELISA Kit; Semcl 2 ELISA Kit; Semcl2 ELISA Kit
缩写：SEMA4D
Uniprot No.：Q92854
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-102 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-100 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-102 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-97 | |||||
回收率：
| The recovery of human sCD100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-106 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80001.8501.8351.8431.75540001.3421.3651.3541.26620000.8340.9000.8670.77910000.5570.5480.5530.4655000.3250.3450.3350.2472500.2530.2650.2590.1711250.1460.1560.1510.06300.0870.0880.088 | ||||||
货期：3-5 working days
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