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产品描述：人臂板蛋白-7A(SEMA7A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SEMA7A含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于神经科学、肿瘤生物学及免疫学等基础研究领域，特别适合用于探究SEMA7A在神经退行性疾病、肿瘤侵袭转移或免疫细胞信号传导中的作用机制，为相关疾病的分子机制研究提供可靠的定量分析工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD 108 ELISA Kit; CD108 ELISA Kit; CD108 antigen ELISA Kit; CDw108 ELISA Kit; H Sema K1 ELISA Kit; H Sema L ELISA Kit; H-SEMA-K1 ELISA Kit; H-Sema-L ELISA Kit; JMH ELISA Kit; JMH blood group antigen ELISA Kit; John Milton Hagen blood group H Sema K1 ELISA Kit; John Milton Hargen human blood group Ag ELISA Kit; John-Milton-Hargen human blood group Ag ELISA Kit; MGC126692 ELISA Kit; MGC126696 ELISA Kit; SEM7A_HUMAN ELISA Kit; Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A ELISA Kit; Sema K1 ELISA Kit; Sema L ELISA Kit; SEMA7A ELISA Kit; SEMAK1 ELISA Kit; SEMAL ELISA Kit; Semaphorin 7A GPI membrane anchor ELISA Kit; Semaphorin K1 ELISA Kit; Semaphorin L ELISA Kit; Semaphorin-7A ELISA Kit; Semaphorin-K1 ELISA Kit; Semaphorin-L ELISA Kit; Semaphorin7a ELISA Kit
缩写：SEMA7A
Uniprot No.：O75326
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 82-96 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 84-98 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of human SEMA7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-94 | ||||
| EDTA plasma (n=4) | 103 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.2462.0892.1681.984101.5961.6921.6441.46051.1231.0681.0960.9122.50.6990.6870.6930.5091.250.4650.4540.4600.2760.6250.3390.3240.3320.1480.3120.2600.2680.2640.08000.1850.1830.184 | ||||||
货期：3-5 working days
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