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产品描述：人臂板蛋白-4A(SEMA4A)酶联免疫试剂盒，定量检测血清、血浆、组织匀浆样本中的SEMA4A含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外定量分析多种生物样本中SEMA4A的浓度变化，可支持癌症机制研究、免疫系统功能探索或神经退行性疾病相关的基础科研实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CORD10 ELISA Kit; RP11 54H19 2 ELISA Kit; RP35 ELISA Kit; SEM4A_HUMAN ELISA Kit; Sema B ELISA Kit; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain 4A ELISA Kit; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4A ELISA Kit; Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) ELISA Kit; Sema4a ELISA Kit; SEMAB ELISA Kit; Semaphorin 4A precursor ELISA Kit; Semaphorin B ELISA Kit; Semaphorin-4A ELISA Kit; Semaphorin-B ELISA Kit; SEMB ELISA Kit
缩写：SEMA4A
Uniprot No.：Q9H3S1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA4A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-100 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 88-95 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human SEMA4A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 83 | 80-86 | ||||
| EDTA plasma (n=4) | 87 | 81-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6042.6352.6202.478101.8631.8991.8811.73951.0211.0361.0290.8872.50.5640.5320.5480.4061.250.3150.3350.3250.1830.6250.2590.2710.2650.1230.3120.2060.1990.2030.06100.1410.1430.142 | ||||||
货期：3-5 working days
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