| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ACTA1; ACTA; Actin; alpha skeletal muscle; Alpha-actin-1 |
| Entrez GeneID | 58 |
| clone | 2A5 |
| WB Predicted band size | Calculated MW: 42 kDa; Observed MW: 42 kDa |
| Host/Isotype | Mouse IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of α skeletal muscle actin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于alpha Skeletal Muscle Actin(ACTA1)抗体的参考文献摘要：
1. **文献名称**: *"ACTA1 mutations and their role in congenital myopathies"*
**作者**: Agrawal PB, et al.
**摘要**: 研究分析了ACTA1基因突变导致的骨骼肌疾病，利用特异性抗体验证突变蛋白在肌肉组织中的异常表达，揭示了突变对肌动蛋白聚合功能的破坏机制。
2. **文献名称**: *"Immunohistochemical characterization of skeletal muscle actin isoforms in human biopsies"*
**作者**: Hnia K, et al.
**摘要**: 比较了多种骨骼肌肌动蛋白抗体(包括ACTA1)在肌肉活检中的特异性，证实其在诊断肌营养不良症和炎症性肌病中的临床应用价值。
3. **文献名称**: *"Structural implications of ACTA1 mutations in nemaline myopathy"*
**作者**: Nowak KJ, et al.
**摘要**: 通过免疫荧光和Western blot结合ACTA1抗体，发现特定突变导致肌动蛋白丝结构缺陷，阐明了杆状体肌病的分子病理机制。
4. **文献名称**: *"Alpha-skeletal muscle actin deficiency in a mouse model"*
**作者**: Laing NG, et al.
**摘要**: 在ACTA1敲除小鼠模型中，利用抗体检测到肌纤维发育异常，揭示了该蛋白在肌肉收缩和胚胎发育中的关键作用。
(注：以上文献标题和作者为示例性内容，实际引用需核对原文信息。)
Alpha-skeletal muscle actin (α-SMA) is a key contractile protein predominantly expressed in skeletal muscle cells, where it forms part of the thin filaments in sarcomeres, enabling muscle contraction. As one of six actin isoforms in mammals, α-SMA belongs to the actin family of cytoskeletal proteins and is encoded by the *ACTA1* gene. It plays a critical role in maintaining skeletal muscle structure, force generation, and mechanotransduction.
Antibodies targeting α-SMA are widely used as research tools to identify and study skeletal muscle cells in developmental biology, regenerative medicine, and disease models. These antibodies are particularly valuable in distinguishing skeletal muscle fibers from smooth or cardiac muscle (which express α-cardiac or β-cytoplasmic actin isoforms) via immunohistochemistry, immunofluorescence, or Western blotting. They are also employed in investigating pathological conditions such as muscular dystrophies, myopathies, and sarcopenia, where α-SMA expression may be altered.
Importantly, some α-SMA antibodies may cross-react with smooth muscle actin (e.g., in myofibroblasts) due to sequence homology, necessitating rigorous validation using controls like knockout tissues or cell lines. Monoclonal antibodies (e.g., clones 1A4. α-sr-1) are commonly used for specificity. Beyond research, α-SMA antibodies have diagnostic applications in differentiating muscle tumor subtypes. Their utility in tracking muscle regeneration, satellite cell activation, and fibrosis underscores their importance in both basic and translational muscle biology.
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