| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H2BK5ac; H2B 1A; H2B; H2B histone family; H2B2f; H2Ba; H2Bf; HIST2H2BF; histone H2B; histone H2B type 1; Histone H2B type 2-F |
| Entrez GeneID | 3018 |
| clone | 4B2 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of Histone H2B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇涉及Histone H2B抗体的参考文献及其核心内容概括：
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1. **文献名称**：*"Histone H2B Monoubiquitination Functions in Chromatin Assembly"*
**作者**：H. Zhou et al.
**摘要**：该研究揭示了H2B单泛素化在染色质组装和DNA损伤修复中的作用，通过特异性抗体验证了H2B泛素化修饰与BRCA1复合体的相互作用。
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2. **文献名称**：*"Antibody-Based Detection of Histone H2B Release in Apoptotic Cells"*
**作者**：K. Samejima et al.
**摘要**：开发了一种针对H2B C端表位的抗体，用于检测细胞凋亡过程中H2B从染色质的释放，证实其作为细胞死亡标志物的潜力。
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3. **文献名称**：*"H2B Ubiquitylation: A Signaling Code for DNA Damage Response?"*
**作者**：M. Nakamura & T. Tokunaga
**摘要**：通过免疫沉淀(IP)和ChIP实验，利用H2B抗体证明H2B泛素化在DNA损伤信号转导中的调控机制，关联ATM/ATR激酶通路。
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这些文献涵盖了H2B抗体在染色质动态、细胞凋亡和DNA损伤研究中的关键应用。如需更多方向(如癌症或表观遗传学)，可进一步补充。
Histone H2B is one of the five core histone proteins (H2A, H2B, H3. H4. and linker histone H1) essential for packaging DNA into chromatin in eukaryotic cells. It forms a heterodimer with H2A, contributing to the nucleosome structure, where two H2A-H2B dimers combine with an H3-H4 tetramer to wrap ~147 base pairs of DNA. H2B plays critical roles in chromatin compaction, transcriptional regulation, DNA repair, and replication. Post-translational modifications (PTMs) of H2B, such as acetylation, ubiquitination, phosphorylation, and methylation, influence chromatin dynamics and epigenetic signaling.
Antibodies targeting Histone H2B are widely used in epigenetics and molecular biology research to study nucleosome organization, chromatin remodeling, and PTM-specific regulatory mechanisms. These antibodies are essential tools for techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry. They help investigate H2B’s involvement in cellular processes, including apoptosis, cell cycle progression, and DNA damage response. Specific H2B antibodies may recognize conserved epitopes across species or modifications like H2B ubiquitination (e.g., K120 in humans), which is linked to transcriptional elongation and DNA repair.
Due to high sequence conservation among H2B variants, antibody specificity must be validated using knockout controls or competitive assays. Dysregulation of H2B expression or PTMs is implicated in cancers and neurodegenerative diseases, making these antibodies valuable for both basic research and translational studies targeting epigenetic abnormalities.
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