| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1 |
| Entrez GeneID | 1973 |
| clone | 6G7 |
| WB Predicted band size | Calculated MW: 46 kDa; Observed MW: 46 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of eIF4A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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1. **"eIF4A1 enhances the Warburg effect and tumor progression through mTOR signaling in triple-negative breast cancer"**
- **作者**: Li et al.
- **摘要**: 研究探讨了eIF4A1在调控三阴性乳腺癌代谢重编程(Warburg效应)中的作用，通过mTOR信号通路促进肿瘤生长，并利用特异性抗体验证其蛋白表达水平与临床预后的相关性。
2. **"Targeting eIF4A1 with rocaglamide derivatives inhibits tumor growth and synergizes with chemotherapy in pancreatic cancer"**
- **作者**: Chu et al.
- **摘要**: 报道了eIF4A1抑制剂Rocaglamide类似物的抗肿瘤效果，通过Western blot和免疫荧光(使用eIF4A1抗体)证明其抑制翻译起始并增强化疗敏感性，为胰腺癌治疗提供新策略。
3. **"The helicase eIF4A1 modulates microRNA-mediated gene silencing in human cells"**
- **作者**: Meijer et al.
- **摘要**: 揭示了eIF4A1通过调控RNA解旋活性影响miRNA介导的基因沉默机制，实验通过siRNA敲低和抗体免疫沉淀证实其与RNA诱导沉默复合物(RISC)的相互作用。
4. **"eIF4A1-dependent mRNA translation regulates neuronal function and survival in Alzheimer's disease models"**
- **作者**: Hernandez et al.
- **摘要**: 研究eIF4A1在阿尔茨海默病模型中的神经保护作用，利用抗体检测其脑组织表达变化，发现其通过调节突触相关蛋白翻译影响神经元存活和认知功能。
(注：上述文献名为示例，实际引用需根据具体研究调整。)
The eukaryotic initiation factor 4A1 (eIF4A1) is a critical component of the translation initiation machinery, belonging to the DEAD-box RNA helicase family. As part of the eIF4F complex, it facilitates the unwinding of secondary structures in mRNA 5'UTRs, enabling ribosome scanning and start codon recognition. Its ATP-dependent RNA helicase activity is essential for protein synthesis, particularly under stress conditions or in mRNAs with complex secondary structures.
eIF4A1 antibodies are widely used in research to study translation regulation, cellular stress responses, and oncogenic pathways. Dysregulation of eIF4A1 is linked to cancers, as many tumors exploit its activity to promote the translation of pro-growth and survival mRNAs. Inhibitors targeting eIF4A1. such as silvestrol, are being explored for anticancer therapies, highlighting its biomedical relevance.
In experimental settings, eIF4A1 antibodies are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess protein expression, subcellular localization, and interactions. Researchers also use these antibodies to investigate its role in diseases beyond cancer, including viral infections and neurodegenerative disorders, where translational control is disrupted. Specificity validation is crucial due to homology with its paralog eIF4A2. requiring antibodies that distinguish between these isoforms.
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