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产品描述：人臂板蛋白-3G(SEMA3G)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SEMA3G含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于基础科研中对SEMA3G表达水平的精准分析；为探索SEMA3G在神经系统发育、肿瘤血管生成或免疫调控中的分子机制提供可靠工具，也可用于评估疾病模型或药物干预下SEMA3G的浓度变化；支持科研人员在体外实验中对SEMA3G进行高通量定量检测，适用于细胞生物学、病理机制研究及生物标志物筛选等非临床研究场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ00014 ELISA Kit; MGC119473 ELISA Kit; Sem2 ELISA Kit; SEM3G_HUMAN ELISA Kit; Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3G ELISA Kit; Sema3g ELISA Kit; Semaphorin 3G ELISA Kit; Semaphorin sem2 ELISA Kit; Semaphorin-3G ELISA Kit
缩写：SEMA3G
Uniprot No.：Q9NS98
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 91-102 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 96-105 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of human SEMA3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 82-91 | |||||
| EDTA plasma (n=4) | 97 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected402.0051.9411.9731.824201.6131.5761.5951.446101.1441.1091.1270.97850.7460.7330.7400.5912.50.5440.5240.5340.3851.250.3830.3720.3780.2290.6250.2750.2690.2720.12300.1520.1460.149 | |||||||
货期：3-5 working days
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