| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB |
| Entrez GeneID | 5970 |
| clone | 3G2 |
| WB Predicted band size | Calculated MW: 60 kDa; Observed MW: 65 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Protein of Transcription factor p65 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3-4条关于NF-κB p65抗体的参考文献示例(内容为模拟虚构，仅供参考)：
1. **文献名称**：*"NF-κB p65 subunit-specific antibody validation in transcriptional regulation studies"*
**作者**：Smith J, et al.
**摘要**：本研究通过Western blot、免疫荧光和ChIP实验验证了NF-κB p65抗体的特异性，证明其在检测细胞核内p65转位及DNA结合活性中的可靠性，为炎症信号通路研究提供关键工具。
2. **文献名称**：*"A comprehensive analysis of NF-κB p65 phosphorylation and subcellular localization using phospho-specific antibodies"*
**作者**：Lee H, et al.
**摘要**：作者开发并验证了针对p65不同磷酸化位点(如Ser536)的特异性抗体，揭示了TNF-α刺激下p65的动力学修饰模式及其与疾病模型的关联。
3. **文献名称**：*"Targeting NF-κB p65 in cancer: Antibody-based inhibition of pro-survival signaling"*
**作者**：Wang Y, et al.
**摘要**：通过使用高亲和力p65抗体阻断肿瘤细胞中NF-κB的活性，研究证明该策略可显著抑制癌细胞增殖并增强化疗敏感性，为靶向治疗提供新思路。
4. **文献名称**：*"Cross-reactivity evaluation of commercial NF-κB p65 antibodies in murine and human models"*
**作者**：Garcia R, et al.
**摘要**：系统评估了多种市售p65抗体的种属交叉反应性，指出部分抗体在小鼠模型中存在非特异性结合问题，强调了抗体验证在跨物种研究中的重要性。
(注：以上文献信息为示例性模拟，实际引用请查询PubMed或学术数据库。)
The NF-κB p65 antibody is a crucial tool for studying the NF-κB signaling pathway, a central regulator of immune responses, inflammation, and cell survival. NF-κB (Nuclear Factor Kappa B) comprises a family of transcription factors, with p65 (RelA) being a major subunit. It typically forms heterodimers with p50. which are sequestered in the cytoplasm by inhibitory proteins like IκB in resting cells. Upon stimulation by cytokines, pathogens, or stress signals, the IκB kinase (IKK) complex phosphorylates IκB, leading to its ubiquitination and proteasomal degradation. This releases p65/p50 dimers, enabling their nuclear translocation to activate target genes involved in immune regulation, apoptosis inhibition, and inflammation.
The p65 antibody specifically detects the RelA subunit, allowing researchers to track NF-κB activation status through techniques like Western blotting, immunofluorescence, or chromatin immunoprecipitation (ChIP). It helps visualize subcellular localization (cytoplasmic vs. nuclear) and quantify expression levels under experimental conditions such as inflammation models, cancer studies, or drug treatments. Due to p65's role in diseases like cancer, autoimmune disorders, and chronic inflammation, this antibody is widely used in both basic research and therapeutic development. Commercial p65 antibodies are often validated for species cross-reactivity (e.g., human, mouse, rat) and application-specific performance to ensure reliability in diverse experimental setups.
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