首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人穿孔素/成孔蛋白(PRF1/PFP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PRF1含量。PRF1即穿孔素1，是一种由细胞毒性淋巴细胞分泌的蛋白质。其在免疫防御中发挥关键作用，可在靶细胞膜上形成孔道，促使颗粒酶等进入细胞，启动细胞凋亡程序，目前在肿瘤免疫治疗等领域为研究机制的重点对象。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于免疫学基础研究、细胞毒性作用机制分析、疾病模型或药物干预下的穿孔素表达水平检测等科研场景，为探索免疫相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cytolysin ELISA Kit; FLH2 ELISA Kit; HPLH2 ELISA Kit; Lymphocyte pore-forming protein ELISA Kit; P1 ELISA Kit; PERF_HUMAN ELISA Kit; perforin 1 (pore forming protein) ELISA Kit; Perforin 1 ELISA Kit; Perforin-1 ELISA Kit; PFP ELISA Kit; PGFL ELISA Kit; PIGF ELISA Kit; PIGF-2 ELISA Kit; PLGF ELISA Kit; Pore forming protein ELISA Kit; prf1 ELISA Kit; SHGC-10760 ELISA Kit
缩写：PRF1
Uniprot No.：P14222
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human PRF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 90 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.7642.6662.7152.602101.9121.9321.9221.80951.3011.3131.3071.1942.50.6890.6570.6730.5601.250.3760.3580.3670.2540.630.2810.2720.2770.1640.3120.1890.1840.1870.07400.1140.1120.113 | ||||||
货期：3-5 working days
×
