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产品描述：人氧还原蛋白过氧化物酶4(PRDX4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PRDX4含量。PRDX4 是具有一定生物学意义的靶点。它在细胞抗氧化防御等方面发挥作用。相关研究聚焦于其参与的信号通路、与疾病发生发展的关联机制，旨在通过揭示其作用原理，为相关疾病如癌症等的治疗提供新靶点和潜在治疗策略。试剂盒检测范围为0.625 ng/mL-40 ng/mL，应用场景包括但不限于探索氧化应激相关疾病（如神经退行性疾病、代谢综合征）的分子机制、评估细胞或动物模型中PRDX4的调控功能，以及通过血清/血浆样本分析PRDX4在特定病理生理条件下的生物学意义。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Antioxidant enzyme 372 ELISA Kit; Antioxidant enzyme AOE372 ELISA Kit; AOE37 2 ELISA Kit; AOE37-2 ELISA Kit; AOE372 ELISA Kit; EC 1.11.1.15 ELISA Kit; Peroxiredoxin IV ELISA Kit; Peroxiredoxin-4 ELISA Kit; Peroxiredoxin4 ELISA Kit; PRDX 4 ELISA Kit; Prdx4 ELISA Kit; PRDX4_HUMAN ELISA Kit; PRX 4 ELISA Kit; Prx IV ELISA Kit; Prx-IV ELISA Kit; PRX4 ELISA Kit; PrxIV ELISA Kit; Thioredoxin dependent peroxide reductase A0372 ELISA Kit; Thioredoxin Peroxidase (Antioxidant Enzyme) ELISA Kit; Thioredoxin peroxidase ELISA Kit; Thioredoxin peroxidase AO372 ELISA Kit; Thioredoxin-dependent peroxide reductase A0372 ELISA Kit; TRANK ELISA Kit
缩写：PRDX4
Uniprot No.：Q13162
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 100-108 | |||||
| 1:4 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 91-97 | |||||
回收率：
| The recovery of human PRDX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-89 | ||||
| EDTA plasma (n=4) | 92 | 86-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.6482.6092.6292.459202.2242.2982.2612.091101.5451.6041.5751.40550.8160.8350.8260.6562.50.5310.5010.5160.3461.250.3250.3170.3210.1510.6250.2410.2600.2510.08100.1690.1710.170 | ||||||
货期：3-5 working days
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