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Rabbit Monoclonal Phospho-CDK1/2/3(Thr14) Antibody

  • 中文名： Phospho-CDK1/2/3 (Thr14)抗体
  • 别    名： CDC2; CDC28A; P34CDC2
货号: IPDX20111
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Hamster
IF 1/20 Human,Hamster
IHC 咨询技术 Human,Hamster
ICC 技术咨询 Human,Hamster
FCM 咨询技术 Human,Hamster
Elisa 咨询技术 Human,Hamster

产品详情

AliasesCDC2; CDC28A; P34CDC2
Entrez GeneID983/1017/1018
WB Predicted band sizeCalculated MW: 34 kDa; Observed MW: 34 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Hamster
ImmunogenA synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk1
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于Phospho-CDK1/2/3 (Thr14)抗体的3篇参考文献及其摘要内容：

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1. **文献名称**: *"Inactivation of CDC2/Cyclin B kinase by WEE1 kinase: a conserved mechanism regulating CDK activity"*

**作者**: Parker, L.L., Piwnica-Worms, H.

**摘要**: 该研究揭示了WEE1激酶通过磷酸化CDK1(Thr14和Tyr15位点)抑制其活性，调控细胞周期G2/M期转换。文中使用Phospho-CDK1 (Thr14)特异性抗体验证了磷酸化修饰的存在及其对CDK1功能的影响。

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2. **文献名称**: *"Phosphorylation of human CDC25B and CDC25C by CDK1/cyclin B: role in modulating subcellular localization"*

**作者**: Gu, Y., Rosenblatt, J., Morgan, D.O.

**摘要**: 作者探讨了CDK1/2/3在Thr14磷酸化如何调控CDC25磷酸酶活性，进而影响细胞周期进程。研究通过Phospho-CDK1/2/3 (Thr14)抗体进行免疫印迹分析，证实该修饰在DNA损伤检查点中的作用。

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3. **文献名称**: *"Cyclin-dependent kinase 1 (CDK1) phosphorylation coordinates Aurora kinase activity during mitosis"*

**作者**: Smits, V.A., Medema, R.H.

**摘要**: 本文发现CDK1 Thr14磷酸化通过抑制其激酶活性，参与有丝分裂中Aurora激酶的动态调控。研究利用Phospho-CDK1 (Thr14)抗体进行免疫荧光实验，揭示了磷酸化水平与染色体分离的关联。

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4. **文献名称**: *"CDK1 and CDK2 activity levels determine the efficiency of CDK inhibitors in human tumor cells"*

**作者**: Santamaría, D., Barrière, C., Cerqueira, A.

**摘要**: 该文献分析了不同CDK抑制剂对肿瘤细胞周期的影响，发现CDK1/2/3 Thr14磷酸化状态可预测药物敏感性。通过Phospho-CDK1/2/3 (Thr14)抗体的流式细胞术检测，验证了磷酸化水平与细胞周期停滞的相关性。

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这些文献均涉及Phospho-CDK1/2/3 (Thr14)抗体的实验应用，涵盖功能机制、疾病模型或药物响应研究。如需具体实验细节，可进一步查阅原文。

背景信息

The Phospho-CDK1/2/3 (Thr14) antibody is designed to specifically detect the phosphorylation status of cyclin-dependent kinases 1. 2. and 3 (CDK1. CDK2. CDK3) at threonine 14 (Thr14), a critical regulatory site involved in modulating their enzymatic activity. CDKs are serine/threonine kinases that play pivotal roles in cell cycle progression, with CDK1 primarily driving the G2/M transition, CDK2 regulating S-phase entry and progression, and CDK3 contributing to G1 phase regulation. Phosphorylation at Thr14. along with Tyr15. is a conserved inhibitory mechanism that suppresses CDK activity by preventing ATP binding. This modification is catalyzed by the Wee1/Myt1 kinase family during the G2 phase, ensuring cell cycle arrest in response to DNA damage or incomplete replication. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25 phosphatases activates CDKs, promoting cell cycle progression. The Phospho-CDK1/2/3 (Thr14) antibody is widely used in research to study cell cycle checkpoints, DNA damage responses, and dysregulated CDK activity in diseases like cancer. It enables the detection of inhibitory phosphorylation events via techniques such as Western blotting, immunofluorescence, or flow cytometry, providing insights into mechanisms underlying cell proliferation, apoptosis, and therapeutic resistance. Specific validation for cross-reactivity and application contexts is essential for accurate interpretation.

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