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产品描述:人过氧化物还原酶6(PRDX6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PRDX6含量。PRDX6是重要的靶点。它是过氧化物酶家族成员,具有抗氧化、磷脂酶等活性。研究机制方面,其在细胞抗氧化应激、膜修复等过程发挥作用,异常表达与多种疾病如肿瘤、肺部疾病等相关,是药物研发潜在靶点。试剂盒检测范围为12.5 pg/mL-800 pg/mL,适用于体外实验中探索PRDX6在肿瘤、心血管疾病或神经退行性疾病中的分子调控机制,也可用于细胞或动物模型中氧化应激相关通路的基础研究。试剂盒提供标准品及完整检测组分,支持实验室开展蛋白质表达分析、药物干预效果评估或病理生理过程研究本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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缩写:PRDX6
Uniprot No.:P30041
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:12.5 pg/mL-800 pg/mL
灵敏度:3.12 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Metabolism
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 84 | ||||
Range % | 80-87 | |||||
1:2 | Average % | 92 | ||||
Range % | 87-96 | |||||
1:4 | Average % | 91 | ||||
Range % | 85-97 | |||||
1:8 | Average % | 97 | ||||
Range % | 91-103 |
回收率:
The recovery of human PRDX6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 100 | 95-105 | ||||
EDTA plasma (n=4) | 97 | 93-102 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2AverageCorrected8002.1442.3022.2232.0984001.7181.7681.7431.6182001.3031.3211.3121.1871000.8470.8680.8580.733500.5350.5610.5480.423250.3520.3420.3470.22212.50.2860.2890.2880.16300.1240.1260.125 |
货期:3-5 working days
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