首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠阿黑皮素原(POMC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的POMC含量。POMC（阿黑皮素原）是一种重要的神经肽前体蛋白。它在人体能量平衡、代谢调节、神经内分泌等方面发挥关键作用。研究机制主要聚焦于其裂解产生的多种活性肽，如促黑素、促肾上腺皮质激素等，参与调节食欲、应激反应等生理过程。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于神经内分泌研究、肥胖机制探索、下丘脑 - 垂体轴功能分析等科研领域，特别适合评估实验动物模型在药物干预、代谢调控或应激状态下POMC的表达水平变化。为研究能量平衡、摄食行为及相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pomc ELISA kit; Pomc2Pro-opiomelanocortin ELISA kit; POMC ELISA kit; Corticotropin-lipotropin) [Cleaved into: NPP; Melanotropin gamma ELISA kit; Gamma-MSH); Potential peptide; Corticotropin ELISA kit; Adrenocorticotropic hormone ELISA kit; ACTH); Melanocyte-stimulating hormone alpha ELISA kit; Alpha-MSH ELISA kit; Melanotropin alpha); Corticotropin-like intermediary peptide ELISA kit; CLIP); Lipotropin beta ELISA kit; Beta-LPH); Lipotropin gamma ELISA kit; Gamma-LPH); Melanocyte-stimulating hormone beta ELISA kit; Beta-MSH ELISA kit; Melanotropin beta); Beta-endorphin; Met-enkephalin] ELISA kit
缩写：POMC
Uniprot No.：P01194
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 99 | ||||
| Range % | 93-105 | |||||
| 1:10 | Average % | 93 | ||||
| Range % | 90-97 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:40 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of rat POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 87 | 85-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.5022.6032.5532.4382501.6611.7621.7121.5971251.1271.1481.1381.02362.50.7240.7130.7190.60431.20.3880.3770.3830.26815.60.2870.2760.2820.1677.80.1840.1810.1830.06800.1150.1140.115 | ||||||
货期：3-5 working days
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