首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人阿黑皮素原(POMC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、组织匀浆样本中的POMC含量。POMC（阿黑皮素原）是一种前体蛋白，在调节能量平衡、体重控制及内分泌等方面发挥重要作用。其背景涉及它在垂体及下丘脑等部位表达。研究机制方面，POMC 经不同酶裂解产生多种生物活性肽，通过与相应受体结合调节机体生理功能。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于科研领域中对POMC表达水平的定量分析，例如探究内分泌调控机制、代谢相关疾病模型中的激素动态变化，或神经科学领域中的神经肽功能研究。产品兼容多种样本类型，可为体外实验提供灵敏可靠的检测方案，助力于分子机制研究及药物开发中的靶点验证。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACTH ELISA Kit; Adrenocorticotropic hormone ELISA Kit; Adrenocorticotropin ELISA Kit; alpha melanocyte stimulating hormone ELISA Kit; Alpha MSH ELISA Kit; Alpha-MSH ELISA Kit; beta endorphin ELISA Kit; Beta LPH ELISA Kit; beta melanocyte stimulating hormone ELISA Kit; Beta MSH ELISA Kit; Beta-LPH ELISA Kit; Beta-MSH ELISA Kit; CLIP ELISA Kit; COLI_HUMAN ELISA Kit; Corticotropin like intermediary peptide ELISA Kit; Corticotropin lipotropin ELISA Kit; Corticotropin-lipotropin ELISA Kit; Gamma MSH ELISA Kit; Gamma-LPH ELISA Kit; Gamma-MSH ELISA Kit; Lipotropin beta ELISA Kit; Lipotropin gamma ELISA Kit; LPH ELISA Kit; Melanotropin alpha ELISA Kit; Melanotropin ELISA Kit; Melanotropin beta ELISA Kit; Melanotropin gamma ELISA Kit; met enkephalin ELISA Kit; Met-enkephalin ELISA Kit; MSH ELISA Kit; NPP ELISA Kit; POC ELISA Kit; POMC ELISA Kit; pro ACTH endorphin ELISA Kit; Proopiomelanocortin ELISA Kit; Proopiomelanocortin preproprotein ELISA Kit
缩写：POMC
Uniprot No.：P01189
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-102 | |||||
回收率：
| The recovery of human POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| EDTA plasma (n=4) | 102 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.8652.8992.8822.70525002.4982.4862.4922.31512502.0042.0562.0301.8536251.5621.5981.5801.4033121.0841.0541.0690.8921560.6920.6820.6870.510780.3240.3570.3410.16400.1740.1790.177 | ||||||
货期：3-5 working days
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