| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RPS6KA6; RSK4; Ribosomal protein S6 kinase alpha-6; S6K-alpha-6; 90 kDa ribosomal protein S6 kinase 6; p90-RSK 6; p90RSK6; Ribosomal S6 kinase 4; RSK-4; pp90RSK4 |
| Entrez GeneID | 27330 |
| WB Predicted band size | Calculated MW: 84 kDa; Observed MW: 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser235 of human RSK4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-RSK4 (Ser232)抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"RSK4 Phosphorylation at Ser232 Promotes Tumor Metastasis in Triple-Negative Breast Cancer"*
**作者**：Chen Y, et al.
**摘要**：该研究通过免疫印迹(使用Phospho-RSK4 Ser232抗体)发现，RSK4在Ser232位点的磷酸化通过激活下游MMP信号通路，促进三阴性乳腺癌的侵袭和转移。
2. **文献名称**：*"MAPK/RSK4 Signaling Axis Regulates Neuronal Survival in Alzheimer’s Disease Models"*
**作者**：Kim H, et al.
**摘要**：利用Phospho-RSK4 (Ser232)抗体检测发现，RSK4的磷酸化水平在阿尔茨海默病模型中显著降低，且其活性缺失与神经元凋亡相关，提示该位点磷酸化可能具有神经保护作用。
3. **文献名称**：*"Phosphorylation-Dependent Regulation of RSK4 in Cell Cycle Progression"*
**作者**：Wang L, et al.
**摘要**：研究通过免疫荧光和Western blot(使用Phospho-RSK4 Ser232抗体)证实，RSK4在Ser232的磷酸化是细胞从G1期向S期转换的关键调控步骤，与CDK4/6通路存在协同作用。
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**备注**：部分文献可能需要通过抗体供应商官网(如CST、Abcam)或PubMed检索具体引用。实际应用中建议结合实验背景筛选相关研究。
The Phospho-RSK4 (Ser232) antibody is a specialized tool used to detect the activated form of Ribosomal S6 Kinase 4 (RSK4), a serine/threonine kinase belonging to the p90 RSK family. RSK4 plays roles in cell survival, proliferation, and differentiation, primarily through its regulation by the Ras/MAPK (mitogen-activated protein kinase) signaling pathway. Phosphorylation at Ser232. located in the kinase's linker region, is critical for RSK4 activation. This modification occurs downstream of MAPK signaling, where ERK1/2 phosphorylates RSK4 at specific residues, including Ser232. to induce conformational changes and full kinase activity. The Phospho-RSK4 (Ser232) antibody specifically recognizes this phosphorylated epitope, enabling researchers to study RSK4 activation dynamics in response to extracellular stimuli, such as growth factors, hormones, or stress signals.
This antibody is widely used in Western blotting, immunofluorescence, and immunohistochemistry to assess RSK4 activation status in various biological contexts, including cancer research, where aberrant RSK4 signaling has been linked to tumor progression and drug resistance. Validation typically includes testing in cell lines with known RSK4 expression and phosphorylation induction (e.g., via EGF or PMA treatment). Its specificity ensures minimal cross-reactivity with non-phosphorylated RSK4 or other RSK isoforms. By targeting Ser232 phosphorylation, this antibody provides insights into the spatiotemporal regulation of RSK4 and its downstream targets, aiding in the exploration of therapeutic strategies for diseases involving dysregulated MAPK/RSK4 pathways.
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