首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠足细胞标记蛋白/足盂蛋白(PCX)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液样本中的PODXL含量。PODXL即足糖萼蛋白，它是一种跨膜蛋白，在胚胎发育等过程发挥作用。研究显示它与肿瘤发生、发展密切相关，可促进肿瘤细胞增殖、迁移和侵袭，还能影响肿瘤血管生成，有望成为癌症诊断标志物和治疗靶点，为癌症防治提供新思路。试剂盒检测范围为1.56 ng/mL-100 ng/mL，科研人员可通过动态监测实验动物或细胞模型中PCX的表达变化，用于探究肾脏疾病发生发展机制、评估药物干预效果或建立体外足细胞损伤模型等基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Podxl ELISA Kit; Pclp1 ELISA Kit; Podocalyxin ELISA Kit; Podocalyxin-like protein 1 ELISA Kit; PC ELISA Kit; PCLP-1 ELISA Kit
缩写：PODXL
Uniprot No.：Q9WTQ2
种属：Rattus norvegicus (Rat)
样本类型：serum, urine, cell culture supernates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PCX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 81-93 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of rat PCX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-97 | ||||
| EDTA plasma (n=4) | 89 | 83-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.2012.3122.2572.112501.5741.4561.5151.370251.0091.0561.0330.88812.50.6330.6540.6440.4996.250.4010.4120.4070.2623.120.2720.2860.2790.1341.560.2100.2150.2130.06800.1440.1460.145 | ||||||
货期：3-5 working days
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