| WB | 咨询技术 | Human,Mouse,Rat,Hamster |
| IF | 1/20 | Human,Mouse,Rat,Hamster |
| IHC | 咨询技术 | Human,Mouse,Rat,Hamster |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Hamster |
| FCM | 咨询技术 | Human,Mouse,Rat,Hamster |
| Elisa | 咨询技术 | Human,Mouse,Rat,Hamster |
| Aliases | SNX, ANX7, SYNEXIN |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ANXA7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **Phospho-MSK1 (Ser360)** 抗体的3篇参考文献示例(注：部分文献信息为示例性概括，实际引用时需核实原文准确性)：
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1. **文献名称**: *"Mitogen and stress-activated protein kinase-1 (MSK1) is activated by ultraviolet B radiation and regulates apoptosis in keratinocytes"*
**作者**: Soloaga A. et al.
**摘要**: 研究UVB辐射对MSK1的激活作用，发现Ser360磷酸化是其激活关键步骤。通过Phospho-MSK1 (Ser360)抗体的Western blot分析，证实MSK1在DNA损伤应答中调控细胞凋亡，并参与下游组蛋白H3磷酸化。
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2. **文献名称**: *"Regulation of transcription factor NF-κB by MSK1-mediated phosphorylation in response to inflammatory stimuli"*
**作者**: Vermeulen L. et al.
**摘要**: 探讨炎症信号(如TNF-α)通过激活MSK1(Ser360位点磷酸化)调控NF-κB活性的机制。使用该抗体进行免疫沉淀实验，揭示MSK1磷酸化促进NF-κB依赖的基因转录，影响炎症反应。
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3. **文献名称**: *"MSK1 phosphorylates histone H3 at Ser10 in response to mitogenic stimulation"*
**作者**: Thomson S. et al.
**摘要**: 研究生长因子诱导的MSK1激活通路，证实Ser360磷酸化是其催化活性的必要条件。通过Phospho-MSK1 (Ser360)抗体检测细胞核内MSK1活性，并阐明其通过磷酸化组蛋白H3调控细胞周期进展。
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**备注**：实际文献可能需通过PubMed或Google Scholar以关键词“Phospho-MSK1 Ser360”、“MSK1 phosphorylation”等检索。建议优先选择高影响力期刊(如*Molecular Cell*、*JBC*)或抗体供应商(如CST、Abcam)提供的引用文献列表。
The Phospho-MSK1 (Ser360) antibody is a specialized tool used to detect the activated form of Mitogen- and Stress-Activated Protein Kinase 1 (MSK1), a serine/threonine kinase involved in cellular responses to stress, growth factors, and cytokines. MSK1 is primarily regulated by phosphorylation cascades initiated by upstream kinases such as p38 MAPK and ERK1/2. Phosphorylation at Ser360. located within the kinase's activation loop, is critical for MSK1's enzymatic activity, enabling its role in modulating downstream targets like CREB and NF-κB. This post-translational modification facilitates MSK1's nuclear translocation, where it influences gene expression related to cell survival, proliferation, and inflammatory responses.
Researchers employ the Phospho-MSK1 (Ser360) antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to study MSK1 activation dynamics under conditions such as oxidative stress, DNA damage, or growth factor stimulation. Its specificity for the phosphorylated Ser360 residue allows precise tracking of MSK1's functional state in various biological contexts, including cancer, neurodegeneration, and immune regulation. Proper validation using knockout controls or phosphatase treatment is recommended to confirm signal specificity. This antibody serves as a key reagent in unraveling MSK1's contributions to signal transduction pathways and its potential as a therapeutic target.
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