首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠磷酸肌醇3激酶(PI3K)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PI3K含量。PI3K（磷脂酰肌醇-3激酶）是一种脂类激酶，在癌症发生发展中起着关键作用。其研究背景源于其在细胞信号传导中的重要作用，特别是在PI3K/Akt/mTOR信号通路中，该通路调控细胞生长、存活、代谢和血管生成。PI3K通过磷酸化脂质分子PI-3,4,5-P3（PIP3）来激活下游信号分子，如Akt和mTOR，从而促进肿瘤生长和抵抗治疗。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品可广泛应用于基础科研领域，包括但不限于肿瘤微环境研究、代谢性疾病模型构建、药物作用机制探索等实验场景，为PI3K相关信号通路分析及分子调控研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PI3K
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-94 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of rat PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 96 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.8602.7782.8192.679 5002.2312.2222.2272.087 2501.3791.4041.3921.252 1250.7480.7610.7550.615 62.50.4640.4480.4560.316 31.20.3070.3020.3050.165 15.60.2180.2110.2150.075 00.1410.1390.140 | ||||||
货期：3-5 working days
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