首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肽酶抑制剂16酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PI16含量。人肽酶抑制剂16是一种新型肽-肽酶抑制剂结合物，通过共价连接肽和肽酶抑制剂，延长肽的血清半衰期，提高药物疗效。其作用机制主要是通过抑制特定肽酶活性，延长肽类药物在体内的作用时间，从而提高治疗效果。试剂盒检测范围为0.312 ng/mL-10 ng/mL，该产品广泛应用于基础科研领域，如炎症性疾病机制研究、感染模型中的宿主防御反应分析、皮肤或肺组织修复过程中的蛋白动态监测等，为探索Elafin在先天免疫调控及病理生理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cripi ELISA Kit; CRISP 9 ELISA Kit; CRISP-9 ELISA Kit; CRISP9 ELISA Kit; Cysteine rich protease inhibitor ELISA Kit; Cysteine rich secretory protein 9 ELISA Kit; Cysteine-rich secretory protein 9 ELISA Kit; Microseminoprotein beta binding protein ELISA Kit; MSMBBP ELISA Kit; Peptidase inhibitor 16 ELISA Kit; PI-16 ELISA Kit; PI16 ELISA Kit; PI16_HUMAN ELISA Kit; Protease inhibitor 16 ELISA Kit; PSP94 binding protein ELISA Kit; PSP94-binding protein ELISA Kit; PSPBP ELISA Kit
缩写：PI16
Uniprot No.：Q6UXB8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-10 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<20% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PI16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 83-98 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 88-105 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 84-105 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 89-100 | ||||||
回收率：
| The recovery of human PI16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-105 | |||||
| EDTA plasma (n=4) | 95 | 86-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 102.2542.2662.260 51.3421.3541.348 2.50.6620.6690.666 0.6250.1970.2110.204 0.3120.0990.1020.101 | |||||||
货期：3-5 working days
×
