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Human Phosphotylinosital 3 kinase,PI3K ELISA Kit

  • 中文名: 人磷酸肌醇3激酶(PI3K)ELISA Kit
货号: IPD-E08417h
Price: ¥2500
数量:
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产品详情

  • 产品描述:人磷酸肌醇3激酶(PI3K)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、组织匀浆、细胞裂解物样本中的PI3K含量。PI3K是重要的信号靶点。其背景在于它在细胞内信号传导中扮演关键角色,与细胞生长、增殖、存活等密切相关。研究机制方面,主要聚焦其激活下游信号通路,如AKT等,异常激活会引发肿瘤等疾病,目前大量研究围绕抑制其活性开展治疗探索。试剂盒检测范围为0.625 ng/mL-40 ng/mL,支持科研人员高效开展PI3K相关的基础研究,例如探索肿瘤发生、免疫调节或代谢性疾病中PI3K信号通路的动态变化,或评估药物干预、基因编辑等实验处理对PI3K表达水平的影响。其宽泛的样本兼容性特别适合多维度实验设计,为体外模型、动物组织或临床前样本中的PI3K定量分析提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA Kit; 5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit; caPI3K ELISA Kit; CLOVE ELISA Kit; CWS5 ELISA Kit; MCAP ELISA Kit; MCM ELISA Kit; MCMTC ELISA Kit; MGC142161 ELISA Kit; MGC142163 ELISA Kit; p110 alpha ELISA Kit; p110alpha ELISA Kit; Phosphatidylinositol 3 kinase catalytic alpha polypeptide ELISA Kit; Phosphatidylinositol 3 kinase catalytic 110 KD alpha ELISA Kit; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha ELISA Kit; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform ELISA Kit; Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha ELISA Kit; Phosphatidylinositol-4 ELISA Kit; Phosphoinositide 3 kinase catalytic alpha polypeptide ELISA Kit; PI 3 Kinase catalytic subunit alpha ELISA Kit; PI3 kinase p110 subunit alpha ELISA Kit; PI3-kinase subunit alpha ELISA Kit; PI3K ELISA Kit; PI3K-alpha ELISA Kit; PI3KC A ELISA Kit; PIK3C A ELISA Kit; Pik3ca ELISA Kit; PK3CA ELISA Kit; PK3CA_HUMAN ELISA Kit; PtdIns 3 kinase p110 ELISA Kit; PtdIns-3-kinase subunit alpha ELISA Kit; PtdIns-3-kinase subunit p110-alpha ELISA Kit; Serine/threonine protein kinase PIK3CA ELISA Kit

  • 缩写:PI3K

  • Uniprot No.:P42336

  • 种属:Homo sapiens (Human)

  • 样本类型:serum, tissue homogenates, cell lysates

  • 检测范围:0.625 ng/mL-40 ng/mL

  • 灵敏度:0.156 ng/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Signal Transduction

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)
    1:1Average %88
    Range %84-98
    1:2Average %98
    Range %91-105
    1:4Average %92
    Range %85-99
    1:8Average %86
    Range %80-92
  • 回收率:

    The recovery of human PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9892-103














  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected402.9422.9002.9212.827202.2892.2782.2842.190101.5761.5451.5611.46750.7880.7680.7780.6842.50.4720.4450.4590.3651.250.2930.2780.2860.1920.6250.2180.2050.2120.11800.0960.0920.094 






  • 货期:3-5 working days

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