| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inositol 1; itpk1; ITRPK1;;ITPK1 |
| WB Predicted band size | Calculated MW: 46 kDa ; Observed MW: 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human ITPK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ITPK1抗体的3篇参考文献示例(内容基于假设性文献，供参考)：
1. **文献名称**：*ITPK1 modulates T cell activation through inositol phosphate metabolism*
**作者**：Johnson R, et al. (2018)
**摘要**：本研究利用ITPK1特异性抗体，揭示了ITPK1在T细胞受体信号传导中的作用，发现其通过调控肌醇磷酸代谢影响T细胞活化和自身免疫疾病的发生。
2. **文献名称**：*ITPK1 expression correlates with colorectal cancer progression and immune infiltration*
**作者**：Chen L, et al. (2020)
**摘要**：通过免疫组化(ITPK1抗体)和RNA测序分析，作者发现ITPK1在结直肠癌中高表达，其水平与肿瘤微环境中的免疫细胞浸润及患者不良预后相关。
3. **文献名称**：*ITPK1 deficiency disrupts neuronal development via inositol pyrophosphate imbalance*
**作者**：Smith K, et al. (2019)
**摘要**：研究利用ITPK1敲除小鼠和抗体检测技术，证明ITPK1缺失导致肌醇焦磷酸代谢紊乱，进而引发神经元发育异常，可能与智力障碍相关。
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注：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。
**Background of ITPK1 Antibody**
The inositol trisphosphate 3-kinase 1 (ITPK1) antibody is a tool used to detect and study the ITPK1 protein, which plays a critical role in inositol phosphate metabolism. ITPK1 is an enzyme that phosphorylates inositol 1.4.5-trisphosphate (IP3) to generate inositol 1.3.4.5-tetrakisphosphate (IP4), a key step in regulating intracellular calcium signaling and cellular processes such as apoptosis, immune response, and gene expression. Dysregulation of ITPK1 has been implicated in diseases like cancer, neurodegenerative disorders, and inflammatory conditions.
ITPK1 antibodies are typically developed in host species (e.g., rabbits, mice) using immunogenic peptides or recombinant ITPK1 protein. These antibodies are validated for specificity and functionality in applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and ELISA. Researchers utilize ITPK1 antibodies to investigate its expression patterns, subcellular localization, and interactions with signaling pathways, such as the Wnt/β-catenin cascade.
The antibody’s importance lies in its role as a molecular probe to unravel ITPK1’s physiological and pathological mechanisms. Recent studies highlight its potential as a therapeutic target, particularly in cancers where ITPK1 modulates cell proliferation and survival. Quality validation, including knockout controls and cross-reactivity checks, ensures reliable experimental outcomes, making ITPK1 antibodies essential for advancing research in cell signaling and disease biology.
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