首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
品描述：大鼠戊糖素(Pentosidine)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Pentosidine含量。Pentosidine是一种晚期糖基化终末产物（AGEs）。它与多种疾病相关，如糖尿病并发症、心血管疾病等。其研究机制主要聚焦在它可介导氧化应激和炎症反应，促进细胞功能障碍和组织损伤，是研究疾病发病机制及寻找潜在治疗靶点的重要方向。试剂盒检测范围为15.6 pmol/mL-1000 pmol/mL，在代谢性疾病机制、氧化应激研究及抗衰老药物开发等科研领域具有重要价值；尤其适用于糖尿病模型大鼠的血清/血浆样本分析，或肝脏、肾脏等组织的匀浆液检测；为研究戊糖素在慢性疾病发展中的作用机制、评估干预措施效果提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Pentosidine
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pmol/mL-1000 pmol/mL
灵敏度：3.9 pmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of rat Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 96-105 | ||||
| EDTA plasma (n=4) | 90 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/mlOD1OD2AverageCorrected10002.0652.0512.0581.9665001.3611.2921.3271.2352500.7450.6970.7210.6291250.3890.4120.4010.30962.50.2190.2330.2260.13431.20.1890.2010.1950.10315.60.1270.1380.1330.04100.0940.0890.092 | ||||||
货期：3-5 working days
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