首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脑啡肽原(PENK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）样本中的PENK含量。PENK是一个靶点。它参与内源性阿片肽系统，与疼痛调节、情绪调控等生理过程相关。研究上，主要聚焦其在疾病发生发展中的作用机制，如在神经精神疾病里的功能变化，探索以它为靶点开发新型治疗药物。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于神经生物学研究、精神疾病动物模型评估、疼痛相关分子机制探索等科研领域。尤其适用于脑脊液等低丰度样本的检测需求，为神经退行性疾病、药物成瘾或应激相关研究提供可靠的定量数据支持，助力科研人员深入解析 PENK 在复杂生理网络中的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Leu-enkephalin ELISA Kit; Met-enkephalin ELISA Kit; Met-enkephalin-Arg-Gly-Leu ELISA Kit; Met-enkephalin-Arg-Phe ELISA Kit; Methionine enkephalin ELISA Kit; OGF ELISA Kit; Opioid growth factor ELISA Kit; PENK ELISA Kit; PENK(114-133) ELISA Kit; PENK(143-183) ELISA Kit; PENK(237-258) ELISA Kit; PENK_HUMAN ELISA Kit; PPA ELISA Kit; Proenkephalin A ELISA Kit; Proenkephalin ELISA Kit; Proenkephalin-A ELISA Kit; Synenkephalin ELISA Kit
缩写：PENK
Uniprot No.：P01210
种属：Homo sapiens (Human)
样本类型：serum, plasma, cerebrospinal fluid (CSF)
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 107 | ||||
| Range % | 98-112 | |||||
回收率：
| The recovery of human PENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 93 | 87-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.3022.4322.3672.2635001.7621.8361.7991.6952501.1141.1961.1551.0511250.6530.6730.6630.55961.50.3750.3850.3800.27631.20.2510.2710.2610.15715.60.1510.1540.1530.04900.1050.1030.104 | ||||||
货期：3-5 working days
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