首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠可溶性血小板内皮细胞粘附分子1(sPECAM-1/sCD31)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PECAM1含量。PECAM1即血小板内皮细胞粘附分子-1，在血管生成、白细胞转运等生理过程中发挥重要作用。研究机制方面，它参与细胞间黏附及信号传导，其异常表达与多种疾病如肿瘤、心血管疾病相关。深入研究有助于了解疾病发病机制并寻找潜在治疗靶点。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于心血管疾病动物模型分析、炎症机制研究以及药物干预效果评价等科研领域，为探索血管内皮功能异常相关疾病提供可靠的实验工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pecam1 ELISA Kit; Pecam ELISA Kit; Platelet endothelial cell adhesion molecule ELISA Kit; PECAM-1 ELISA Kit; CD antigen CD31 ELISA Kit
缩写：PECAM1
Uniprot No.：Q3SWT0
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 86-95 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of rat sPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-98 | |||||
| EDTA plasma (n=4) | 97 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 2002.4682.5542.5112.372 1001.6561.7321.6941.555 501.0331.0671.0500.911 250.5910.6110.6010.462 12.50.3800.3980.3890.250 6.250.2780.2860.2820.143 3.120.2030.2070.2050.066 00.1380.1390.139 | |||||||
货期：3-5 working days
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