首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人戊糖素(Pentosidine)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、尿液样本中的Pentosidine含量。Pentosidine是一个靶点，它属于晚期糖基化终末产物（AGEs）。在体内，它主要通过美拉德反应形成并逐渐积累。研究机制方面，它与糖尿病并发症、衰老等密切相关，可引发氧化应激和炎症反应，常作为评估相关疾病风险和进程的生物标志物。试剂盒检测范围为31.25 pmol/mL-2000 pmol/mL，该产品适用于基础医学研究、疾病机制探索、药物干预效果评估等科研场景，为代谢性疾病、衰老相关疾病及骨关节健康研究提供可靠的生物标志物检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Pentosidine
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, urine
检测范围：31.25 pmol/mL-2000 pmol/mL
灵敏度：7.81 pmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 81-102 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 84-105 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 91-111 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 80-103 | |||||
回收率：
| The recovery of human Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-105 | ||||
| EDTA plasma (n=4) | 94 | 88-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/mlOD1OD2AverageCorrected20002.6772.6972.6872.53710001.8431.8641.8541.7045001.1631.1531.1581.0082500.7420.7540.7480.5981250.4470.4610.4540.30462.50.3470.3590.3530.20331.250.2680.2620.2650.11500.1540.1460.150 | ||||||
货期：3-5 working days
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