首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人色素上皮衍生因子(PEDF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、组织匀浆样本中的PEDF含量。PEDF（色素上皮衍生因子）是一种具有多种生物学功能的分泌性糖蛋白，最初被识别为视网膜色素上皮细胞中的神经营养因子，现被认为是有效的内源性抗血管生成因子。研究表明，PEDF对多种肿瘤细胞具有抑制作用，如卵巢癌、胰腺癌、骨肉瘤等。其作用机制包括靶向肿瘤异常的微血管系统、直接抑制肿瘤细胞增殖或介导其凋亡。由于PEDF分子量较大，半衰期短，临床转化应用存在一定困难，因此，对其衍生肽段的研究具有潜在价值。试剂盒检测范围为0.156 ng/mL-10 ng/mL，在糖尿病视网膜病变、肿瘤微环境调控及神经退行性疾病等研究中具有重要价值；适用于低丰度样本的定量分析；满足实验室对PEDF表达水平的基础研究需求，例如在疾病机制探索中评估不同病理条件下PEDF的分泌变化，或在药物开发中监测体外模型对PEDF表达的影响；为心血管疾病、代谢综合征相关研究以及生物标志物筛选提供可靠的技术支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cell proliferation-inducing gene 35 protein ELISA Kit; EPC 1 ELISA Kit; EPC-1 ELISA Kit; EPC1 ELISA Kit; OI12 ELISA Kit; OI6 ELISA Kit; PEDF ELISA Kit; PEDF_HUMAN ELISA Kit; PIG 35 ELISA Kit; PIG35 ELISA Kit; Pigment epithelium derived factor ELISA Kit; Pigment epithelium-derived factor ELISA Kit; Proliferation inducing protein 35 ELISA Kit; Serine (or cysteine) proteinase inhibitor ELISA Kit; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 ELISA Kit; Serpin F1 ELISA Kit; Serpin family F member 1 ELISA Kit; Serpin peptidase inhibitor ELISA Kit; Serpin peptidase inhibitor clade F member 1 ELISA Kit; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 ELISA Kit; SERPINF 1 ELISA Kit; Serpinf1 ELISA Kit
缩写：PEDF
Uniprot No.：P36955
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, urine, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 96-104 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-104 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-97 | |||||
回收率：
| The recovery of human PEDF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5352.5752.5552.44152.0122.0322.0221.9082.51.3981.4281.4131.2991.250.8470.8670.8570.7430.6250.4780.4980.4880.3740.3120.3360.3460.3410.2270.1560.2370.2500.2440.13000.1090.1180.114 | ||||||
货期：3-5 working days
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