首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠色素上皮衍生因子(PEDF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PEDF含量。PEDF（色素上皮衍生因子）是一种具有多种生物功能的分泌性糖蛋白，主要在视网膜色素上皮细胞中表达。它具有抗血管生成、抗炎和神经营养作用，对多种肿瘤细胞具有抑制作用。其研究机制涉及通过调节细胞增殖、凋亡和迁移等途径抑制肿瘤生长。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于体外培养细胞分泌功能评估、疾病模型动物血清标志物分析以及组织微环境中PEDF动态变化的科研探索，为血管生成调控、氧化应激机制及神经退行性疾病等基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PEDF
Uniprot No.：Q80ZA3
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 87 | ||||
| Range % | 81-92 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4000 | Average % | 92 | ||||
| Range % | 85-97 | |||||
| 1:8000 | Average % | 96 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of rat PEDFspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-95 | ||||
| EDTA plasma (n=4) | 87 | 83-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.7582.6152.6872.5492002.0651.9682.0171.8791001.3571.3971.3771.239500.8460.8310.8390.701250.5720.5810.5770.43912.50.3540.3340.3440.2066.250.2640.2560.2600.12200.1390.1370.138 | ||||||
货期：3-5 working days
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