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产品描述：人Mimecan(OGN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OGN含量。OGN（Osteoglycin，骨糖蛋白）是一种蛋白聚糖类物质，属于富亮氨酸低分子蛋白聚糖（SLRPs）家族。它主要存在于骨骼和软骨中，参与骨骼生长和修复过程。OGN的研究涉及其在细胞信号传导、细胞外基质构建和骨代谢中的作用机制。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于探索Mimecan在心血管疾病模型、代谢调控研究或组织再生机制中的生物学功能；广泛应用于基础科研中的蛋白表达分析、信号通路验证及生物标志物探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Corneal keratan sulfate proteoglycan ELISA Kit; DKFZP586P2421 ELISA Kit; MIME_HUMAN ELISA Kit; Mimecan ELISA Kit; Mimecan proteoglycan ELISA Kit; OG ELISA Kit; OGN ELISA Kit; OIF ELISA Kit; Osteoglycin ELISA Kit; Osteoinductive factor ELISA Kit; SLRR3A ELISA Kit
缩写：OGN
Uniprot No.：P20774
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:20 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:40 | Average % | 99 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of human OGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-104 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4322.3322.3822.25451.8041.7041.7541.6262.51.1871.2871.2371.1091.250.7160.7260.7210.5930.6250.4470.4570.4520.3240.3120.3450.3550.3500.2220.1560.2270.2370.2320.10400.1290.1260.128 | ||||||
货期：3-5 working days
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