| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hUba3; NEDD8 activating enzyme E1C; uba3; UBE1C;;UBA3 |
| WB Predicted band size | Calculated MW: 52 kDa ; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human UBA3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与UBA3抗体相关的参考文献(虚拟示例，实际文献需通过学术数据库检索)：
1. **文献名称**：*UBA3-mediated protein ubiquitination regulates colorectal cancer stemness*
**作者**：Zhang Y, et al.
**摘要**：该研究利用特异性UBA3抗体验证了UBA3在结直肠癌干细胞中的高表达，并发现其通过调控β-catenin泛素化影响肿瘤干性。抗体应用于Western blot和免疫组化实验。
2. **文献名称**：*Development of a monoclonal antibody targeting UBA3 for neurodegenerative disease research*
**作者**：Lee S, et al.
**摘要**：报道了一种高特异性小鼠抗人UBA3单克隆抗体的开发，通过ELISA和免疫共沉淀验证其结合活性，并应用于阿尔茨海默病模型中的UBA3蛋白定位分析。
3. **文献名称**：*UBA3 inhibition suppresses tumor growth by modulating NEDDylation pathways*
**作者**：Wang X, et al.
**摘要**：研究采用UBA3抗体进行组织芯片分析，发现UBA3在多种实体瘤中过表达，并通过靶向NEDDylation通路抑制剂验证其作为治疗靶点的潜力。
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**提示**：实际文献建议通过PubMed/Google Scholar检索关键词 "UBA3 antibody" "UBA3 immunohistochemistry" 或结合具体研究领域筛选。部分商业抗体公司(如CST、Abcam)官网也会提供相关文献引用。
The UBA3 antibody is a crucial tool for studying the ubiquitin-like modifier-activating enzyme 3 (UBA3), a key component of the NEDD8-activating enzyme (NAE) complex. UBA3. in conjunction with APPBP1 (also known as NAE1), forms the heterodimeric E1 enzyme responsible for activating NEDD8. a ubiquitin-like protein. This activation is essential for the neddylation pathway, a post-translational modification process that regulates the activity of cullin-RING ligases (CRLs), which are critical for ubiquitination-mediated protein degradation. Dysregulation of neddylation has been implicated in various cancers, neurodegenerative diseases, and immune disorders, making UBA3 a target of therapeutic interest.
UBA3 antibodies are widely used in research to detect protein expression, localization, and interactions in cells or tissues. They enable techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate UBA3's role in cell cycle progression, DNA repair, and tumorigenesis. Studies have shown that UBA3 overexpression correlates with cancer proliferation and poor prognosis, highlighting its potential as a biomarker or drug target. Inhibitors targeting the NAE complex, such as MLN4924 (Pevonedistat), are under clinical investigation, further emphasizing the relevance of UBA3 in translational research. Researchers rely on high-specificity UBA3 antibodies to unravel molecular mechanisms and evaluate therapeutic interventions in disease models.
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