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产品描述：人牙成釉细胞相关蛋白(ODAM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ODAM含量。人牙成釉细胞相关蛋白(ODAM)是一种在牙齿发育中起关键作用的蛋白，其研究机制涉及调控成釉细胞分化过程，通过单细胞测序技术揭示了其时空表达模式，对牙齿发育和釉质生成具有重要意义。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于口腔生物学、发育生物学等领域的基础研究，例如探究牙齿发育机制、口腔疾病动物模型中ODAM的动态表达规律，或分析矿化组织相关蛋白调控网络等科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ODAM ELISA Kit; APIN ELISA Kit; Odontogenic ameloblast-associated protein ELISA Kit; Apin ELISA Kit
缩写：ODAM
Uniprot No.：A1E959
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.04 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ODAM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 104 | ||||
| Range % | 99-109 | |||||
| 1:10 | Average % | 98 | ||||
| Range % | 95-102 | |||||
| 1:20 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human ODAM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.6822.7902.7362.56452.0792.0052.0421.8702.51.3101.2541.2821.1101.250.7730.7290.7510.5790.6250.4580.4230.4410.2690.3120.3740.3670.3710.1990.1560.2570.2690.2630.09100.1710.1730.172 | ||||||
货期：3-5 working days
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