| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Imp9; Imp9a; Imp9b; Ipo9; RanBP9;;Importin 9 |
| WB Predicted band size | Calculated MW: 116 kDa ; Observed MW: 140 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Importin 9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Importin9/RANBP9抗体的3篇参考文献示例(注：文献为假设性示例，实际引用需核实)：
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1. **文献名称**：*"RANBP9 regulates nuclear import of Smad proteins in TGF-β signaling"*
**作者**：Zhang Y, et al.
**摘要内容**：本研究利用RANBP9特异性抗体进行免疫共沉淀和核质分离实验，揭示了RANBP9通过介导Smad蛋白的核转运调控TGF-β信号通路的机制，并发现其异常表达与肿瘤转移相关。
2. **文献名称**：*"RANBP9 interacts with the amyloid precursor protein and modulates its processing in Alzheimer's disease"*
**作者**：Wang L, et al.
**摘要内容**：通过Western blot和免疫荧光技术结合RANBP9抗体，研究发现RANBP9与淀粉样前体蛋白(APP)直接结合，促进β-分泌酶活性，加剧Aβ肽的生成，提示其在阿尔茨海默病中的病理作用。
3. **文献名称**：*"Importin9/RANBP9 is essential for early embryonic development through mediating ribosomal protein transport"*
**作者**：Tanaka K, et al.
**摘要内容**：使用Importin9抗体进行胚胎干细胞实验，发现其通过调控核糖体蛋白的核输入维持胚胎早期发育，敲除后导致胚胎致死，揭示了其在细胞增殖中的关键功能。
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**注意**：以上文献为示例，实际研究中请通过PubMed或Google Scholar以关键词“RANBP9 antibody”或“Importin9 antibody”检索最新或高引文献，并确认实验方法中是否明确使用该抗体。
The Importin9/RANBP9 antibody targets a multifunctional adaptor protein involved in diverse cellular processes. RANBP9 (Ran-binding protein 9), also known as Importin9. belongs to the importin β superfamily and plays roles in nucleocytoplasmic transport, signaling pathways, and cytoskeletal organization. It interacts with Ran GTPase, a key regulator of nuclear import/export, suggesting its involvement in classical nuclear transport mechanisms governed by the Ran-GTP gradient. Structurally, RANBP9 contains SPRY, LISH, and CRA domains that mediate interactions with partners like growth factor receptors (e.g., EGFR), cell cycle regulators, and tumor suppressors (e.g., p53).
Antibodies against RANBP9/Importin9 are vital tools for studying its localization, expression, and molecular interactions. They are widely used in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to explore its roles in cancer progression, viral infection (e.g., HIV), and neurodegenerative diseases (e.g., Alzheimer’s). Dysregulation of RANBP9 is linked to tumorigenesis, metastasis, and synaptic dysfunction, making it a potential therapeutic target. Researchers also investigate its isoform-specific functions and post-translational modifications, which may influence antibody specificity. Commercial antibodies vary in performance depending on epitope regions and experimental conditions, necessitating validation via knockout controls or siRNA-based approaches. Overall, these antibodies provide critical insights into RANBP9’s dual roles in transport and signaling networks.
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