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产品描述：人8-羟基鸟嘌呤DNA糖苷酶酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆、细胞裂解物样本中的OGG1含量。OGG1是一种重要的DNA修复酶，在维持基因组稳定性方面发挥关键作用。它能特异性识别并切除氧化损伤的鸟嘌呤。研究聚焦于其修复机制及与疾病关联，如癌症、神经退行性疾病等，了解它或有助于开发相关治疗策略。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品可广泛应用于体外氧化损伤模型评估、DNA修复机制研究、疾病相关分子通路探索等科研领域，为研究衰老、肿瘤发生及环境毒素导致的DNA损伤提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：8 hydroxyguanine DNA glycosylase ELISA Kit; 8 oxoguanine DNA glycosylase 1 ELISA Kit; 8 oxoguanine DNA glycosylase ELISA Kit; AP lyase ELISA Kit; DNA (apurinic or apyrimidinic site) lyase ELISA Kit; DNA apurinic or apyrimidinic site lyase ELISA Kit; DNA lyase ELISA Kit; DNA-(apurinic or apyrimidinic site) lyase ELISA Kit; HMMH ELISA Kit; HOGG 1 ELISA Kit; HOGG1 ELISA Kit; MMH ELISA Kit; MUTM ELISA Kit; N glycosylase ELISA Kit; Ogg 1 ELISA Kit; Ogg1 ELISA Kit; OGG1_HUMAN ELISA Kit; OGH 1 ELISA Kit; OGH1 ELISA Kit
缩写：OGG1
Uniprot No.：O15527
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human hOGG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 103 | ||||
| Range % | 98-112 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human hOGG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.7372.8722.8052.654102.0962.1152.1061.95551.4831.4961.4901.3392.50.8720.8390.8560.7051.250.5610.5240.5430.3920.6250.3480.3320.3400.1890.3120.2570.2610.2590.10800.1450.1570.151 | ||||||
货期：3-5 working days
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