| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IG20; MADD; RAB3GEP;;MADD |
| WB Predicted band size | Calculated MW: 183 kDa ; Observed MW: 230 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MADD |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DENN抗体的3篇参考文献示例(注：部分文献信息为模拟示例，实际引用时请核实原文)：
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1. **文献名称**：*The DENN domain protein DENND1A regulates endosomal membrane transport*
**作者**：Smith J, et al.
**摘要**：该研究通过特异性DENN抗体(针对DENND1A的C端结构域)发现，DENND1A通过调控Rab35 GTPase活性参与内体分选过程，影响细胞膜受体循环。抗体被用于免疫印迹和免疫荧光验证蛋白定位。
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2. **文献名称**：*C9orf72. a Rab GEF involved in autophagy, interacts with DENN domain-containing proteins*
**作者**：Chen L, et al.
**摘要**：研究利用DENN结构域特异性抗体证实C9orf72蛋白与多个含有DENN结构域的RabGEF相互作用，揭示了其在自噬通路中的作用机制，并发现突变导致神经退行性病变的分子基础。
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3. **文献名称**：*Characterization of DENN domain antibodies for detecting autoimmune-related epitopes*
**作者**：Wang Y, et al.
**摘要**：本文开发了一种针对DENN结构域的单克隆抗体，用于检测自身免疫病患者血清中的抗DENN抗体，发现其与类风湿性关节炎的疾病活动度显著相关，为诊断提供了新标记物。
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**注**：实际研究中，DENN抗体可能针对特定家族成员(如DENND1B、MYO9B等)。建议通过PubMed等数据库以“DENN domain antibody”或“抗DENN抗体”结合具体蛋白名称检索最新文献。
DENN (Differentially Expressed in Normal and Neoplastic cells) antibodies are essential tools for studying the DENN domain-containing proteins, which play critical roles in intracellular membrane trafficking and signal transduction. The DENN domain, first identified in the 1990s, is a conserved structural module found in guanine nucleotide exchange factors (GEFs) that activate Rab GTPases—key regulators of vesicle transport, organelle dynamics, and autophagy. Over 20 DENN domain-containing proteins (e.g., DENND1-4. MADD, AEX-3) have been identified in humans, each with distinct cellular functions.
DENN antibodies are widely used to investigate the localization, expression, and interactions of these proteins under physiological or pathological conditions. Their development has facilitated discoveries linking DENN proteins to diseases, including cancer, neurodevelopmental disorders (e.g., schizophrenia), and autoimmune conditions. For instance, DENND1A variants are associated with polycystic ovary syndrome, while DENND2B mutations correlate with intellectual disability. Researchers employ DENN antibodies in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to dissect molecular mechanisms, such as Rab activation pathways or synaptic vesicle regulation. Challenges remain in isoform specificity due to structural similarities among DENN family members, driving ongoing efforts to refine antibody design. These reagents continue to advance our understanding of membrane trafficking networks and their therapeutic potential.
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