首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠的牛血清白蛋白残留检测酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BSA含量。靶点研究旨在发现疾病过程中的特定分子或细胞结构，通过药物与靶点相互作用产生治疗效果。BSA（牛血清白蛋白）作为一种常用实验材料，在免疫实验中常用作封闭剂，其研究背景涉及靶点的发现、筛选和验证，研究机制则包括基因组学、蛋白质组学和生物信息学等多学科技术。试剂盒检测范围为0.312 μg/mL-80 μg/mL，灵敏度为0.312 μg/mL。适用于生物制品生产流程质控、实验动物血清样本中异源蛋白残留分析，以及生物医药研究中BSA清除工艺的优化验证本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：BSA
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 μg/mL-80 μg/mL
灵敏度：0.312 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 90-110 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 89-106 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 92-100 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 92-99 | ||||||
回收率：
| The recovery of rat BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-102 | |||||
| EDTA plasma (n=4) | 96 | 89-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| μg/mlOD1OD2Average 800.2810.2890.285 200.4450.4380.442 50.5710.5850.578 1.250.7860.7990.793 0.3121.2331.2211.227 01.7781.7951.787 | |||||||
货期：3-5 working days
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