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Rabbit Monoclonal Phospho-PTS(S19) Antibody

  • 中文名: Phospho-PTS(S19)抗体
  • 别    名: PTP synthase; PTPS; PTS;;p-PTS (S19)
货号: IPDX19022
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCDHN; NCAD; CD325; CDw325; CDH2
Entrez GeneID1000
clone5D5
WB Predicted band size99.8kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Rabbit,Monkey
ImmunogenPurified recombinant fragment of human CDH2 expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于Phospho-PTS(S19)抗体的虚构参考文献示例,供参考:

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1. **文献名称**: *Phosphorylation of PTS at Serine 19 Modulates Apoptotic Signaling in Cancer Cells*

**作者**: Smith A, et al.

**摘要**: 本研究通过Western blot和免疫荧光技术,利用Phospho-PTS(S19)抗体证实了PTS蛋白在Ser19位点的磷酸化与细胞凋亡通路相关。实验表明,该修饰可增强PTS与Bcl-2家族蛋白的相互作用,从而调控线粒体依赖性凋亡。

2. **文献名称**: *Role of PTS(S19) Phosphorylation in MAPK Pathway Activation*

**作者**: Johnson B, et al.

**摘要**: 文章揭示了PTS蛋白在Ser19位点的磷酸化通过激活MAPK信号通路促进细胞增殖。作者使用Phospho-PTS(S19)抗体进行免疫沉淀实验,证明磷酸化PTS与Raf-1激酶的结合是通路激活的关键步骤。

3. **文献名称**: *Development and Validation of a Phospho-Specific Antibody for PTS(S19)*

**作者**: Chen C, et al.

**摘要**: 本文描述了一种高特异性的Phospho-PTS(S19)多克隆抗体的制备方法,并通过ELISA、肽竞争实验和多种细胞模型验证其应用。结果显示该抗体在检测肿瘤组织中PTS激活状态具有高灵敏度和特异性。

4. **文献名称**: *PTS Phosphorylation at Ser19 Correlates with Poor Prognosis in Glioblastoma*

**作者**: Lee D, et al.

**摘要**: 通过对胶质母细胞瘤患者样本的免疫组化分析(使用Phospho-PTS(S19)抗体),研究发现Ser19磷酸化水平与肿瘤侵袭性和患者生存率显著相关,提示其作为潜在预后标志物的价值。

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注:以上文献为虚构示例,实际研究中请通过PubMed、Google Scholar等平台检索真实文献。

背景信息

The Phospho-PTS(S19) antibody is designed to detect the phosphorylated form of a protein at serine 19 (S19) within a phosphotransferase system (PTS)-related sequence. PTS systems are primarily associated with bacterial carbohydrate transport and phosphorylation, but homologous motifs exist in eukaryotic proteins, where they may regulate nutrient sensing, metabolic signaling, or stress responses. Phosphorylation at S19 is hypothesized to modulate protein activity, localization, or interactions in pathways linked to cellular energy homeostasis or signal transduction. This antibody enables researchers to study the dynamic phosphorylation status of the target protein in experimental models, particularly in contexts like metabolic disorders, cancer (where altered phosphorylation often drives pathogenesis), or microbial studies. It is commonly validated via Western blot, immunofluorescence, or immunoprecipitation, with specificity confirmed using phosphorylation-deficient mutants or phosphatase-treated samples. Its applications span basic research in cell biology, microbiology, and translational studies exploring phosphorylation-dependent mechanisms in disease.

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