| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PAM |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于AMSH抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*AMSH interacts with ESCRT-0 to regulate the sorting and degradation of ubiquitinated membrane proteins*
**作者**：Itoh T, et al.
**摘要**：该研究通过抗AMSH抗体的免疫共沉淀实验，揭示了AMSH与ESCRT-0复合物的相互作用，调控泛素化膜蛋白在内体系统中的分选与降解过程，强调了AMSH在溶酶体途径中的关键作用。
2. **文献名称**：*Structural basis for selective recognition of Lys63-linked polyubiquitin chains by the AMSH protease*
**作者**：Sato Y, et al.
**摘要**：利用抗AMSH抗体进行蛋白纯化和功能分析，阐明了AMSH蛋白酶通过其N端结构域特异性识别K63连接泛素链的分子机制，为去泛素化酶底物选择性提供结构依据。
3. **文献名称**：*Regulation of epidermal growth factor receptor down-regulation by AMSH-mediated deubiquitination*
**作者**：McCullough J, et al.
**摘要**：通过抗AMSH抗体的功能抑制实验，证明AMSH通过去泛素化修饰调控EGFR的内吞和溶酶体降解，揭示了其在受体酪氨酸激酶信号下调中的关键角色。
(注：以上文献信息为示例，实际引用需根据具体文献核实作者、标题及摘要内容。)
AMSH (Associated Molecule with the SH3 domain of STAM) is a cytosolic protein involved in regulating intracellular signaling and endosomal sorting. It interacts with STAM (Signal Transducing Adaptor Molecule), a component of the ESCRT (Endosomal Sorting Complex Required for Transport) machinery, to modulate lysosomal trafficking and receptor tyrosine kinase signaling, such as EGFR degradation. AMSH contains an N-terminal MIT (Microtubule Interacting and Trafficking) domain, enabling binding to ubiquitinated substrates, and a C-terminal SH3-binding motif. Functionally, AMSH acts as a deubiquitinating enzyme (DUB) belonging to the JAMM/MPN+ metalloprotease family, selectively cleaving K63-linked polyubiquitin chains. This activity regulates protein stability, signal transduction, and membrane receptor recycling.
Research highlights its role in cancer, where altered AMSH expression correlates with tumor progression, metastasis, and drug resistance. Overexpression in certain cancers (e.g., breast, ovarian) may influence cell proliferation and apoptosis. Additionally, AMSH is studied in reproductive health as a biomarker for ovarian reserve, where its levels in serum reflect follicular development and are used to assess fertility potential in assisted reproductive technologies. Its dual role in cellular homeostasis and disease pathways makes AMSH a potential therapeutic target. Commercial antibodies targeting AMSH are widely used in Western blotting, immunohistochemistry, and ELISA to investigate its expression, localization, and functional mechanisms in both physiological and pathological contexts.
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