首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胃泌酸调节素酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OXM含量。OXM（胰高血糖素样肽 -1/胰高血糖素双受体激动剂）具有独特背景。它涉及调节能量代谢、血糖的生理机制。研究表明，OXM可作用于相应受体，促进胰岛素分泌、抑制胰高血糖素分泌、延缓胃排空，有望用于肥胖、糖尿病等疾病的治疗。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于大鼠模型中OXM表达水平与胃肠功能、代谢调控的相关性研究，可应用于药物干预、饮食调控或病理状态下OXM动态变化的科研分析。样本处理简便，标准曲线覆盖生理及病理浓度范围，满足基础研究中血清、血浆等液态样本及胃肠组织匀浆的检测需求，为消化系统与代谢调控领域的分子机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：OXM
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of rat oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-100 | ||||
| EDTA plasma (n=4) | 99 | 95-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.3562.3782.3672.25240002.1212.1612.1412.02620001.7571.7981.7781.66310001.3431.3911.3671.2525000.8510.8990.8750.7602500.5020.5390.5210.4061250.2720.2910.2820.16700.1140.1160.115 | ||||||
货期：3-5 working days
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