首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠神经肽Nesfatin-1酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Nesfatin-1含量。Nesfatin-1是一种由核组蛋白2（NUCB2）衍生而来的肽段，主要在下丘脑和外周组织表达，具有抑制摄食、调节能量代谢、参与生长发育和胰岛素抵抗等生物学功能。其研究机制涉及调节下丘脑黑皮质素信号通路、影响食欲、降低血糖、促进胰岛素分泌和调节糖代谢中酶的活性等方面。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于神经生物学、代谢调控机制研究，以及肥胖相关动物模型的病理机制探索；为研究Nesfatin - 1在生理及病理过程中的作用提供可靠工具；适用于实验室规模的样本批量检测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Nesfatin-1
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rat nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 97-109 | ||||
| EDTA plasma (n=4) | 97 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7952.6522.7242.5735001.8571.7761.8171.6662501.0041.0821.0430.8921250.6540.6130.6340.48362.50.4240.4300.4270.27631.20.3170.2990.3080.15715.60.2260.2090.2180.06700.1540.1470.151 | ||||||
货期：3-5 working days
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