首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠高密度脂蛋白胆固醇酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HDL-C含量。HDL - C即高密度脂蛋白胆固醇，它被视作“好胆固醇”，背景上与心血管健康密切相关，较高水平的HDL - C有助于降低心血管疾病风险。其研究机制主要是促进胆固醇逆向转运，将外周组织中的胆固醇转运到肝脏进行代谢，减少胆固醇沉积。试剂盒检测范围为93.75 ng/mL-6000 ng/mL，适用于动脉粥样硬化发病机制研究、脂代谢调控实验、药物干预效果评价等科研领域，可支持动物模型构建、营养学实验及心血管相关分子通路的探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：HDL-C
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 ng/mL-6000 ng/mL
灵敏度：23.4 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 83-89 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-102 | |||||
| 1:800 | Average % | 90 | ||||
| Range % | 85-93 | |||||
| 1:1600 | Average % | 99 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of rat HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 107 | 101-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected60002.6122.5542.5832.48330002.1222.1582.1402.04015001.4491.4531.4511.3517500.8450.8310.8380.7383750.4200.4340.4270.327187.50.2720.2780.2750.17593.750.1760.1690.1730.07300.1010.0990.100 | ||||||
货期：3-5 working days
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