| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GRB4; NCK adaptor protein 2; NCK2; NCKbeta;;NCK2 |
| WB Predicted band size | Calculated MW: 43 kDa ; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NCK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Nck2抗体的参考文献示例(文献信息为虚构，供参考)：
1. **文献名称**：*Nck2 regulates dendritic spine formation through interactions with neuronal signaling pathways*
**作者**：Chen L, et al.
**摘要**：研究利用特异性Nck2抗体揭示其在神经元突触发育中的作用，证实Nck2通过调控WAVE复合体促进树突棘形态发生。
2. **文献名称**：*Characterization of a monoclonal Nck2 antibody for functional studies in cancer cell migration*
**作者**：Rodriguez M, et al.
**摘要**：开发并验证了一种高特异性抗Nck2单克隆抗体，证明其在乳腺癌细胞中抑制Nck2与受体酪氨酸激酶(如EGFR)的相互作用可降低细胞迁移能力。
3. **文献名称**：*Nck2 deficiency alters vascular development in mouse models revealed by antibody-based profiling*
**作者**：Sato K, et al.
**摘要**：通过Nck2抗体免疫组化分析，发现Nck2基因敲除小鼠血管内皮细胞形态异常，提示其在血管生成中调控细胞骨架重排的关键功能。
4. **文献名称**：*A phospho-specific Nck2 antibody identifies its role in T-cell receptor signaling*
**作者**：Gupta R, et al.
**摘要**：利用磷酸化特异性Nck2抗体，揭示T细胞激活过程中Nck2的酪氨酸磷酸化位点对下游信号通路的调控机制。
(注：以上文献为示例性质，实际研究需参考真实数据库如PubMed。)
The Nck2 antibody is a crucial tool in studying the Nck adaptor protein family, particularly Nck2 (also known as Grb4). Nck2 is a cytosolic adaptor protein involved in intracellular signaling pathways, facilitating protein-protein interactions through its SH2 and SH3 domains. It plays a role in coordinating signals from cell surface receptors, such as receptor tyrosine kinases, to downstream effectors regulating cell motility, cytoskeletal reorganization, and axon guidance. Nck2 shares structural homology with Nck1 but exhibits distinct expression patterns and functional roles, with Nck2 being more prominently expressed in the brain and involved in neuronal development.
Antibodies targeting Nck2 are widely used to investigate its expression, localization, and interactions in various biological contexts. They enable detection of Nck2 in techniques like Western blotting, immunoprecipitation, and immunofluorescence, helping researchers dissect its role in diseases, including cancer and neurological disorders. For instance, Nck2 dysregulation has been linked to tumor progression and metastasis, making its antibody valuable in cancer research. Specific Nck2 antibodies are validated for species reactivity (e.g., human, mouse, rat) and application-specific efficacy. Researchers rely on these antibodies to explore Nck2's interplay with signaling molecules like PAK kinases, Eph receptors, and WAVE complexes, advancing understanding of cellular communication networks. Quality validation (e.g., knockout controls) ensures antibody specificity, minimizing off-target effects in experimental models.
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